Objective To investigate the neuroprotective effect of Jian Nao Zeng Zhi Yin on Alzheimer's disease(AD)cell model and its effect on apoptosis.Methods PC12 cells were treated with 20 μmol/L amyloid-β(Aβ1)-42 for 24 h and divided into six groups:normal cell control group(NC group),AD cell model group(AD group),positive drug control AD group(AD+Donepezil group)and Jian Nao Zeng Zhi Yin(low concentration,medium concentration and high concentration)treatment AD group(AD+5 μg/ml Jian Nao Zeng Zhi Yin group,AD+10 μg/ml Jian Nao Zeng Zhi Yin group,AD+20 μg/ml Jian Nao Zeng Zhi Yin group).Cell viability was detected by CCK-8 assay;the expression levels of apoptotic factor-related proteins Bax and Bcl-2 were detected by Western blotting;and apoptosis was determined by flow cytometry.Results The cellular activity was tested by CCK-8 assay,and the results showed that the cellular activity in AD group was significantly decreased compared with the NC group(P＜0.05);compared with the AD group,the cellular activity in AD+Donepezil group,AD+20 μg/ml Jian Nao Zeng Zhi Yin group was also significantly increased(P＜0.05).The expression levels of apoptotic factor-related proteins were tested by western blotting,and the results showed that the expression level of Bax was significantly up-regulated,Bcl-2 was significantly down-regulated and Bcl-2/Bax was significantly down-regulated in AD group compared with the NC group(P＜0.05);compared with the AD group,Bax in AD+Donepezil group,AD+10 μg/ml Jian Nao Zeng Zhi Yin group and AD+20 μg/ml Jian Nao Zeng Zhi Yin group was significantly down-regulated,while Bcl-2/Bax in AD+Donepezil group and AD+20 μg/ml Jian Nao Zeng Zhi Yin group was significantly up-regulated(P＜0.05).The apoptosis was tested by flow cytometry,and the results showed that the apoptosis rate was(7.870±0.241)%in NC group,(36.530±1.429)%in AD group,(10.690±1.257)%in AD+Donepezil group,(26.930±1.790)%in AD+5 μg/ml Jian Nao Zeng Zhi Yin group,(23.800±1.136)%in AD+10 μg/ml Jian Nao Zeng Zhi Yin group,(10.410±1.903)%in AD+20 μg/ml Jian Nao Zeng Zhi Yin group.Compared with the NC group,the apoptosis rate of AD group was significantly increased(P＜0.05);compared with the AD group,the apoptosis rate of AD+5 μg/ml Jian Nao Zeng Zhi Yin group,AD+10 μg/ml Jian Nao Zeng Zhi Yin group and AD+20 μg/ml Jian Nao Zeng Zhi Yin group was significantly decreased(P＜0.05).Conclusion Jian Nao Zeng Zhi Yin may reduce apoptosis of AD cell and increase cell viability by regulating the expression of Bcl-2/Bax protein,and play a neuroprotective role in AD cell model.High concentration(20 μg/ml)of Jian Nao Zeng Zhi Yin treatment group has a better efficacy,which can provide a theoretical and clinical basis for the further exploration of the traditional Chinese medicine treatment of cognitive disorders.
维普
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