Effect analysis of allogeneic blood transfusion induced macrophage dysdifferentiation on wound healing in diabetic foot ulcer patients
Objective To investigate the effect of macrophage dysdifferentiation induced by allogeneic blood transfusion on wound healing in diabetic foot ulcer patients.Methods 90 patients with macrophage dysdifferentiation induced by allogeneic blood transfusion were divided into diabetic foot ulcer group and foot trauma ulcer group according to the causes of ulcers,each with 45 cases.After admission,all patients underwent bacterial culture + drug sensitivity test.According to the results of bacterial culture,the patient was treated with antibiotics.Meanwhile,repeated debridement was performed until the wound was fresh and the granulation tissue was well grown.The foot function score,number of inflammatory infiltrating cells,message ribonucleic acid(mRNA)relative expression levels of interleukin-12β(IL-12β),inducible nitric oxide synthase(iNOS)and tumor necrosis factor-α(TNF-α)in wound tissue of two groups before and after intervention were compared.Results Before intervention,there were no significant differences in American Orthopaedic Foot and Ankle Society(AOFAS)score and AOFAS function score between diabetic foot ulcer group and foot trauma ulcer group(P>0.05).After intervention,the AOFAS score and AOFAS functional score of diabetic foot ulcer group and foot trauma ulcer group were higher than those before intervention,and the statistical difference was significant(P<0.05).After intervention,the AOFAS score and AOFAS function score of diabetic foot ulcer group were(62.53±4.21)and(42.14±3.21)points,which were lower than(89.68±6.51)and(48.37±4.59)points of traumatic foot ulcer group,and the statistical difference was significant(P<0.05).Before intervention,there was no significant difference in the number of inflammatory infiltrating cells between diabetic foot ulcer group and foot trauma ulcer group(P>0.05).After intervention,the number of inflammatory infiltrating cells in diabetic foot ulcer group and foot trauma ulcer group was lower than that those before intervention,and the statistical difference was significant(P<0.05).After intervention,the number of inflammatory infiltrating cells in diabetic foot ulcer group was(41.33±4.09)/high-power field,which was higher than(33.21±1.75)/high-power field in foot trauma ulcer group,and the statistical difference was significant(P<0.05).Before intervention,there was no significant difference in the mRNA relative expression levels of IL-12β,iNOS and TNF-α between diabetic foot ulcer group and foot trauma ulcer group(P>0.05).After intervention,the mRNA relative expressions of IL-12β,iNOS and TNF-α in diabetic foot ulcer group and foot trauma ulcer group were lower than those before intervention,and the statistical difference was significant(P<0.05).The mRNA relative expression levels of IL-12β,iNOS and TNF-α in the diabetic foot ulcer group were(0.068±0.012),(56.822±12.116)and(1.812±0.246),which were higher than(0.025±0.011),(33.032±11.005)and(0.833±0.115)in the foot trauma ulcer group after intervention,and the statistical difference was significant(P<0.05).Conclusion Macrophage dysdifferentiation induced by allogeneic blood transfusion can affect the foot function,the count of inflammatory infiltrating cells in the wound tissue,and mRNA relative expression levels of IL-12β,iNOS and TNF-α in the wound tissue,which should be paid sufficient attention in clinic.
万方数据
维普
