Application of immunohistochemistry in lung biopsy of small cell lung cancer and non-small cell lung cancer
Objective Based on the pathological diagnosis,the expression of multiple molecular markers by immunohistochemistry in differentiating small cell lung cancer from non-small cell lung cancer on lung biopsy.Methods Lung puncture biopsy specimens,fiberoptic bronchoscopy biopsy specimens and pathological data of 13 small cell lung cancer and 47 non-small cell lung cancer patients were analyzed,and the expression levels of multiple molecular markers such as CK,CK7,Napsin A,P40,P63,CgA,Syn,CD56,TTF-1,and Ki-67 were detected in tissues of pathological specimens by immunohistochemistry.The positive expression rates of lung cancer molecular markers were compared between patients with non-small cell lung cancer and small cell lung cancer,and the diagnostic efficacy of lung cancer molecular markers in small cell lung cancer(CgA,Syn,CD56,Ki-67)and non-small cell lung cancer(P40,P63,Napsin A)was analyzed.Results The positive expression rates of CgA,Syn,CD56,Ki-67 and TTF-1 in small cell lung cancer patients were 92.3%,92.3%,100.0%,92.3%and 92.3%,which were higher than 4.2%,2.1%,4.2%,6.4%and 34.0%in non-small cell lung cancer patients;the positive expression rates of P40,P63 and Napsin A in small cell lung cancer patients were 0,0 and 0,which were lower than 59.6%,55.3%and 25.5%in non-small cell lung cancer patients;there were significant differences(P<0.05).The difference in CK7 positive expression rate between non-small cell lung cancer patients and small cell lung cancer patients was not statistically significant(P>0.05).The CK positive expression rate of non-small cell lung cancer patients and small cell lung cancer patients was both 100.0%.The sensitivity of CgA,Syn,CD56 and Ki-67 in diagnosing small cell lung cancer was 0.923,0.923,1.000,0.923,the specificity was 0.957,0.979,0.957,0.936,and the accuracy was 0.950,0.967,0.967,0.933.P63,P40 and Napsin A showed high positivity in non-small cell lung cancer.The sensitivity of P40,P63 and Napsin A in the diagnosis of non-small cell lung cancer was 0.596,0.553 and 0.255,and the specificity was 1.000,1.000 and 1.000,and the accuracy was 0.683,0.650 and 0.417.Conclusion Pathological diagnosis combined with immunohistochemistry to detect the expression levels of P63,P40 and Napsin A has high reliability in the diagnosis of non-small cell lung cancer;while pathological diagnosis combined with immunohistochemistry to detect the expression levels of CgA,Syn,CD56 and Ki-67 has high reliability in the diagnosis of small cell lung cancer.Immunohistochemical detection can provide a reference basis for the pathological diagnosis of both small cell lung cancer and non-small cell lung cancer,and is worthy of clinical promotion and application.
Small cell lung cancerNon-small cell lung cancerImmunohistochemistryLung biopsy
万方数据
维普
