Study on effect of gestational diabetes mellitus on immune function of neonates infected with group B Streptococcus
Objective To explore the effect of gestational diabetes mellitus(GDM)on immune function of neonates infected with group B Streptococcus(GBS).Methods Of the 102 GBS-infected neonates,48 neonates whose mothers had GDM were included in the maternal GDM neonatal infection group,and the remaining 54 neonates whose mothers had normal glucose monitoring during pregnancy were included in the maternal non-GDM neonatal infection group,while 52 neonates with simple hyperbilirubinemia hospitalized were selected as the non-infected control group.Blood samples were collected at the acute stage of disease onset,and the levels of lymphocyte subsets,natural killer(NK)cells,and cytokines were measured by flow cytometry.The levels of immune parameters were compared among the three groups of neonates,and the correlation between NK cells and lymphocyte subset was analyzed.Results The CD3+,CD4+,CD8+,CD4+/CD8+,CD3-CD56+CD16+ cells in maternal GDM neonatal infection group were significantly lower than those in maternal non-GDM neonatal infection group and non-infected control group;the maternal non-GDM neonatal infection group was lower than non-infected control group;the difference was statistically significant(P<0.05).The levels of IL-6 and IL-6/IL-10 in maternal GDM neonatal infection group were higher than those in maternal non-GDM neonatal infection group;the levels of IL-6 and IL-10 in maternal GDM neonatal infection group and maternal non-GDM neonatal infection group were higher than those in non-infected control group;the level of IL-6/IL-10 in maternal GDM neonatal infection group was higher than that in the non-infected control group.The difference was statistically significant(P<0.05).There was no significant difference in IL-10 level between maternal GDM neonatal infection group and maternal non-GDM neonatal infection group(P>0.05).There was no significant difference in IL-6/IL-10 levels between maternal non-GDM neonatal infection group and the non-infected control group(P>0.05).Pearson correlation analysis showed that CD3-CD56+CD16+NK cells were positively correlated with CD3+,CD4+ and CD8+ T cells(r=0.588,0.580,0.461;P<0.05).Conclusion Neonates born to mothers with GDM show more pronounced impaired immune function and proinflammatory/anti-inflammatory dysfunction in the presence of infections;therefore,the prevention and treatment of GDM has a positive significance in improving the immune function of the neonate and improving the prognosis of neonatal infectious diseases.
NeonatesGestational diabetes mellitusGroup B streptococcus infectionT lymphocytesNK cellsCytokine
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