Effects of lncRNA LINC00963 on the proliferation,migration,and invasion of colorectal cancer cells by targeting miR-148b-3p
Colorectal cancer(CRC)is the most common malignant tumor worldwide.Previous experiments have shown that in the exosomes secreted by CRC cells under hypoxic conditions,long non-coding RNA nuclear enriched abundant transcript 1(LncRNA NEAT1)has a significantly higher expression level.An LncRNA NEAT1 of unknown function has been identified by high-throughput transcriptome sequencing in exosomes secreted by CRC cells(HCT116 and SW480)after 48 h of incubation in a hypoxic environment,and its content was significantly up-regulated in exosomes secreted by HCT116 and SW480 in a hypoxic environment.The customized LncRNA NEAT1 mimic was used to treat HCT116 and SW480 cells cultured under normal conditions in vitro,and it was found that LncRNA NEAT1 mimic treatment significantly promoted the proliferation and migration of HCT116 and SW480 cells.The current study has showed that the Si-LINC00963+anti-miR-NC group had a decreased expression level of miR-148b-3p and an increased number of migrating and invasive cells compared with the Si-LINC00963+anti-miR-NC group(P<0.05).It indicated that miR-148b-3p was target-regulated by LINC00963 and that inhibition of miR-148b-3p expression reversed the effects of interfering with LINC00963 on HT29 cells.This study provides new molecular targets for early diagnosis and targeted therapy of CRC,and provides a preclinical research basis for the clinical translation of CRC diagnosis and treatment.
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