Study on influence of arecoline on NF-κB/TGF-β1 signaling pathways in oral submucous fibrosis
Objective To explore the influence of arecoline on Nuclear factor-κB/Transforming growth factor-β1(NF-κB/TGF-β1)signaling pathways in oral submucous fibrosis(OSF).Methods 10 samples of normal oral mucosa of the upper jaw were randomly divide into an observation group and a control group,each with 5 samples.After cell culture,the 6th generation oral mucosal fibroblasts(OMFb)of the observation group were supplemented with arecoline solution at a concentration of 40 μg/ml,and the control group was supplemented with DMEM culture solution containing 10%fetal bovine serum.NF-κB,TGF-β1 mRNA expression,protein content and cell proliferation after 12,24 and 48 h were compared between the two groups.Results The mRNA expressions of NF-κB and TGF-β1 in the observation group were(2.04±0.31)and(1.54±0.22),which were significantly higher than(1.47±0.23)and(1.17±0.18)in the control group,and the difference was statistically significant(P<0.05).The protein contents of NF-κB and TGF-β1 in the observation group were(123.56±3.56)and(90.14±4.15)kDa,which were significantly higher than(77.45±2.14)and(54.11±2.18)kDa in the control group,and the difference was statistically significant(P<0.05).The number of cell proliferation in the observation group were(320.82±48.59)×109 and(380.45±37.85)×109 after 24 and 48 h,which were significantly lower than(600.76±45.37)×109 and(1200.98±100.95)×109 in the control group,and the difference was statistically significant(P<0.05).Conclusion High concentrations of arecoline may promote oral submucosal fibrosis through up-regulation of NF-κB and TGF-β1 pathways,which is of great significance for subsequent clinical studies.
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