小儿肺炎支原体感染采用MRD检测的临床价值研究
Clinical value of MRD detection for Mycoplasma pneumoniae infection in children
李文静 1董广兴1
作者信息
摘要
目的 观察临床微生物快速培养(MRD)检验诊断小儿肺炎支原体感染的有效性及可行性.方法 85 例疑似小儿肺炎支原体感染患儿,均行快速双抗原夹心酶联免疫吸附试验(ELISA)与MRD检测.观察两种方法对小儿肺炎支原体感染的检验结果;以病理检验结果为金标准,比较两种方法对小儿肺炎支原体感染的诊断效能;比较患儿家属对两种方法的满意度.结果 病理结果:85 例疑似小儿肺炎支原体感染患儿中,阳性 75 例,阴性 10 例;ELISA检验结果:阳性 66 例,阴性 19 例;MRD检验结果:阳性 70 例,阴性 15 例.MRD对小儿肺炎支原体感染诊断的特异度 80.00%、灵敏度 90.67%、准确率 89.41%、阳性预测值 97.14%、阴性预测值 53.33%均高于ELISA的 20.00%、77.33%、70.59%、87.88%、10.53%(P<0.05).患儿家属对MRD的总满意率为 95.29%,显著高于ELISA的 85.88%(P<0.05).结论 MRD检测对小儿肺炎支原体感染具备较高的诊断效能,值得临床进一步推广应用.
Abstract
Objective To observe the effectiveness and feasibility of microbial rapid detection(MRD)in diagnosing Mycoplasma pneumoniae infection in children.Methods 85 children with suspected Mycoplasma pneumoniae infection were tested by enzyme-linked immunosorbent assay(ELISA)and MRD.The results of pathological examination were used as the gold standard to compare the diagnostic efficacy of the two methods for mycoplasma pneumoniae infection in children,and the satisfaction of parents with the two methods.Results Pathological results:of 85 children with suspected Mycoplasma pneumoniae infection,75 cases were positive and 10 cases were negative.ELISA test results:66 cases were positive and 19 cases were negative;MRD test results:70 cases were positive and 15 cases were negative.The specificity of MRD for the diagnosis of Mycoplasma pneumoniae infection in children was 80.00%,the sensitivity was 90.67%,the accuracy was 89.41%,the positive predictive value was 97.14%,and the negative predictive value was 53.33%,which were higher than 20.00%,77.33%,70.59%,87.88%and 10.53%of ELISA(P<0.05).The overall satisfaction rate of parents with MRD was 95.29%,which was significantly higher than 85.88%of ELISA(P<0.05).Conclusion MRD detection has high diagnostic efficacy for Mycoplasma pneumoniae infection in children,and is worthy of further clinical application.
关键词
小儿肺炎支原体感染/酶联免疫吸附试验/微生物快速培养Key words
Mycoplasma pneumoniae infection in children/Enzyme linked immunosorbent assay/Microbial rapid detection引用本文复制引用
出版年
2024
NETL
NSTL
万方数据