Epidemiological and antibiotic resistance studies of CRE isolated from different clinical specimens
Objective To understand the epidemiology and drug resistance of carbapenem-resistant enterobacteriaceae(CRE)in local medical institutions,and to provide scientific basis for the prevention and treatment of CRE infection in local medical institutions.Methods VITEK2 Compact was used to identify pathogenic bacteria and drug sensitivity test of clinical specimens from routine microbiological examination,and the strains of CRE were selected and further drug resistance analysis was carried out,finally,the epidemiological characteristics and drug resistance of CRE were analyzed.Results A total of 19 298 microbiological specimens were collected,and 91 strains of CRE were isolated after removing duplicate,including 24 strains(26.4%)of Escherichia coli and 46 strains(50.5%)of Klebsiella pneumoniae,14 strains(15.4%)of Enterobacter cloacae,and 7 other strains(7.7%).Among 91 CRE strains,38 strains(41.8%)produced serine A,52 strains(57.1%)produced metalloenzyme B,and 1 strain(1.1%)produced mixed serine and metalloenzyme.Sputum specimens had the highest probability of CRE isolation,accounting for 49.5%(45/91),and the specimens from the respiratory department had the highest probability of CRE isolation,accounting for 26.4%(24/91);the sensitivities to CRE for amikacin and tigecycline were the highest,accounting for 53.8%(49/91).Conclusion Respiratory department is the main department of CRE infection,and the rate of CRE isolation from sputum is the highest.The main resistance mechanism of CRE in this hospital is the production of metalloenzyme B,and Klebsiella pneumoniae is the main CRE,amikacin and tigecycline are still available for the treatment of CRE infection.