Lnc RNA SNHG14 promotes the development of ovarian cancer by regulating miR-206 expression
Objective To explore the expression of long chain non-coding RNA(Lnc RNA)small nuclear RNA host gene 14(SNHG14)in ovarian cancer tissue and the impact of Lnc RNA SNHG14 on the proliferation,migration,and invasion ability of ovarian cancer through micro RNA(miR)-206.Methods The cancer tissues and their adjacent paracancerous tissues(>3 cm away from the cancer tissues)of 58 cases of patients undergoing radical surgery for ovarian cancer were collected for cell culture,and transfected with negative control plasmid(si-NC group)and SNHG14 high expression plasmid(si-SNHG14 group)when the degree of cell fusion reached about 70%.Quantitative real-time fluorescent polymerase chain reaction(qRT-PCR)was used to detect the expression levels of SNHG14 and miR-206 in ovarian cancer tissues and their adjacent paracancerous tissues;the cell counting kit-8(CCK-8)assay,plate clone formation assay,and Matrigel invasion assay were used to detect the proliferation,clone formation,migration,and invasion of SNHG14 on ovarian cancer cells;the dual luciferase assay was used to detect the targeting relationship between Lnc RNA SNHG14 and miR-206.Results Compared with(1.01±0.08)and(1.00±0.07)in paracancerous tissues,SNHG14 expression of(2.56±0.57)was elevated and miR-206 expression of(0.36±0.11)was decreased in ovarian cancer tissues(P<0.05).The correlation between SNHG14 and miR-206 in ovarian cancer tissues detected by Pearson's method showed that SNHG14 was negatively correlated with miR-206(r=-0.803,P<0.01).Compared with(1.01±0.06)and(1.00±0.08)in the si-NC group,the expression level of SNHG14[(0.27±0.07)]was decreased and the expression level of miR-206[(2.96±0.45)]was increased in the si-SNHG14 group(P<0.05).The si-SNHG14 group had lower optical density(OD)of(0.29±0.05)than(0.79±0.06)in si-NC group,and lower clone formation of(52.35±7.37)than(120.51±19.34)in si-NC group(P<0.05).The number of migrating cells and invading cells were(43.11±5.87)and(49.66±11.01)in si-SNHG14 group,which were significantly lower than(117.11±10.48)and(122.30±12.97)in si-NC group(P<0.05).Starbase was used to predict miR-206 that binds complementarily to SNHG14.Transfection of miR-206 mimics reduced the luciferase activity of ovarian cancer cells containing wild-type vectors(P<0.05)and failed to inhibit the luciferase activity of ovarian cancer cells containing mutant vectors(P>0.05).Conclusion The expression of Lnc RNA SNHG14 is increased in ovarian cancer tissue and ovarian cancer cells.Lnc RNA SNHG14 can target miR-206,thereby regulating the proliferation,clone formation,migration,and invasion of ovarian cancer cells.
Long chain non-coding RNASmall nucleolar RNA host gene 14Micro RNA-206Ovarian cancer cellsProliferationClone formationMigrationInvasion
NETL
NSTL
万方数据
