Determination of six components in Chaiyin Granules by UPLC-wavelength switching method
Objective An ultra performance liquid chromatography(UPLC)-wavelength switching method ias established for the simultaneous determination of six components(chlorogenic acid,lignoceroside,baicalin,baicalein,wogonoside and wogonin)in Chaiyin Granules.Methods The chromatographic column was Shim-pack C18(100 mm×2.1 mm×1.9 μm),and a gradient elution with acetonitrile-0.1%phosphoric acid was adopted at the flow rate of 0.3 ml/min.The detection wavelength were set at 327 nm for chlorogenic acid and luteoloside and at 275 nm for baicalin,wogonoside,baicalein and wogonin),and the column temperature was set at 35℃,the sample size was 5 μl.Results The linear ranges were 12.12-121.22 μg/ml for chlorogenic acid(r=0.9999),2.07-20.74 μg/ml for lignoceroside(r=0.9998),10.56-105.57 μg/ml for baicalin(r=0.9998),1.55-15.48 μg/ml for wogonoside(r=0.9999),2.16-21.63 μg/ml for baicalein(r=0.9997),1.21-12.13 μg/ml for wogonin(r=0.9996).Average recoveries were 95.62%(RSD=2.55%,n=6),94.59%(RSD=2.16%,n=6),93.66%(RSD=1.78%,n=6),96.04%(RSD=3.66%,n=6),94.66%(RSD=2.85%,n=6),93.92%(RSD=2.46%,n=6),respectively.Conclusion The result of UPLC-wavelength switching method is accurate,reliable and has good repeatability,which provideds an effective determination method for the quality control of Chaiyin Granules.
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