Effect analysis of electrochemiluminescence immunoassay and enzyme-linked immunosorbent assay in hepatitis B virus serologic testing
Objective To compare the effect of electrochemiluminescence immunoassay(ECLIA)and enzyme-linked immunosorbent assay(ELISA)in hepatitis B virus serologic testing.Methods 90 patients with hepatitis B were subjected to virus serological testing using electrochemiluminescence immunoassay and enzyme-linked immunosorbent assay.The two diagnostic methods were compared for the positive detection rate of hepatitis B virus[hepatitis B virus core antibody(HBcAb),hepatitis B virus surface antibody(HBsAb),hepatitis B e antigen(HBeAg),hepatitis B virus surface antigen(HBsAg),and hepatitis B virus e antibody(HBeAb)]as well as the reproducibility of the two tests for HBsAg at different concentrations.Results The positive detection rates of electrochemiluminescence immunoassay for HBcAb,HBsAb,HBeAg,HBsAg,HBeAb were 85.56%,33.33%,48.89%,31.11%,64.44% respectively;the positive detection rates of enzyme-linked immunosorbent assay for HBcAb,HBsAb,HBeAg,HBsAg,HBeAb were 83.33%,32.22%,45.56%,27.78%,44.44%,respectively.There was no statistically significant difference in the comparison of the positive detection rates of HBcAb,HBsAb,HBeAg and HBsAg between the two tests(P>0.05),and the positive detection rate of HBeAb by electrochemiluminescence immunoassay was significantly higher than that by enzyme-linked immunosorbent assay(P<0.05).The reproducibility between batches of high concentration HBsAg was similar(P>0.05).In contrast,the reproducibility of electrochemiluminescence immunoassay was significantly lower than that of enzyme-linked immunosorbent assay within batches of high-concentration HBsAg as well as between batches and within batches of medium-and low-concentration HBsAg.Conclusion Electrochemiluminescence immunoassay is the preferred choice for virus serological testing in patients with hepatitis B.It has the advantages of high detection rate and accurate results,and is worthy of clinical use and promotion.