Application value analysis of microcolumn gel assay in the identification of RhD and ABO blood group antigens
Objective To explore the application value of microcolumn gel assay in the identification of RhD and ABO blood group antigens.Methods 120 patients requiring RhD and ABO blood group identification were divided into two portions and analyzed for blood group using the tube method and the manual microcolumn gel immunoassay(microcolumn gel assay),respectively.The results of the two methods were compared.Results Blood samples of 120 patients were tested by tube method and microcolumn gel assay,and 112 cases were found to be ABO positive(34 cases of type A,33 cases of type B,11 cases of type AB,34 cases of type O),the positive rate of ABO was 93.33% .1 case was RhD negative,and the RhD negative rate was 0.83% .The compliance rate of antigen detection between the two methods was 100.00%,and there was no significant difference in blood group detection between the two methods(P>0.05).Conclusion Microcolumn gel assay is simple and accurate in the identification of RhD and ABO blood group antigens.It is worthy of clinical application.
Microcolumn gel assayTube methodABO blood group systemRhD systemAntigen identification