Comparison of the diagnostic value of enzyme-linked immunosorbent assay and real-time fluorescence quantitative PCR for hepatitis B
Objective To compared the diagnostic value of enzyme-linked immunosorbent assay and real-time fluorescence quantitative polymerase chain reaction(PCR)for hepatitis B.Methods A total of 120 patients with suspected hepatitis B were selected as the subjects of this study,and were examined by enzyme-linked immunosorbent assay and real-time fluorescence quantitative PCR respectively.Using pathological examination as the gold standard,the results of enzyme-linked immunosorbent assay and real-time fluorescence quantitative PCR were analyzed,and the diagnostic efficacy of the two methods was compared,including specificity,sensitivity and accuracy.Results 75 of the 120 suspected patients were confirmed as hepatitis B by pathological examination.Enzyme-linked immunosorbent assay detected 74 positive cases and 46 negative cases,while real-time fluorescence quantitative PCR detected 75 positive cases and 45 negative cases.The specificity,sensitivity and accuracy of real-time fluorescence quantitative PCR for detecting hepatitis B were 93.33%,96.00%and 95.00%,which were significantly higher than 73.33%,82.67%and 79.17%of enzyme-linked immunosorbent assay.The difference was statistically significant(χ2=6.4800,6.9963,13.3724;P<0.05).Conclusion Real-time fluorescence PCR detection of hepatitis B patients has a high accuracy,and can provide a good basis for doctors to make diagnosis and treatment plan and adjustment.
万方数据
维普
