Study on the effect of sample preservation time on the results of electrochemiluminescence detection of tacrolimus concentration
Objective To explore the effect of sample preservation time on the results of electrochemiluminescence detection of tacrolimus concentration. Methods The whole blood samples from 168 patients treated with tacrolimus were selected as observation subjects,and were divided into Group A (48 cases),Group B (30 cases),Group C (30 cases),Group D (30 cases),and Group E (30 cases) according to the different preservation times of the samples. Tacrolimus concentration was detected by electrochemiluminescence in all patients. Group A was immediately tested by adding to a EP tube after pre-treatment on the day of blood collection,while Group B was tested by adding to a EP tube at room temperature for 30 min after pre-treatment,Group C was tested by adding a EP tube at room temperature for 60 min after pre-treatment,Group D was pre-treated and placed in a 2-8 ℃ refrigerator for 3 d before testing,and Group E was pre-treated and placed in a 2-8 ℃ refrigerator for 7 d before testing. The results of Group A were used as the standard to compare the concentration of tacrolimus detected in Group B,Group C,Group D and Group E. Results The concentrations of tacrolimus detected in Group A,Group B,Group C,Group D and Group E were (7.92±1.26),(7.96±1.36),(8.58±1.48),(8.01±1.19) and (8.66±1.56) ng/ml,respectively. There was no statistically significant difference in the concentration of tacrolimus in whole blood specimens of Group B and Group D compared with Group A (P>0.05). The concentration of tacrolimus in whole blood samples of Group C and Group E were higher than those of Group A,and the difference was statistically significant (P<0.05). Conclusion In the process of clinical electrochemiluminescence testing,it is necessary to pay attention to the management of specimen preservation time,especially for specific drugs (such as tacrolimus). It is recommended to complete all operations within a short period of time (30 min) after obtaining the test sample,and it is important to note that the time should not be too long (≤ 60 min);if the current test environment and operation can not meet the requirements,then it is recommended that the test samples be refrigerated. If the current test environment and operation can not meet the requirements,it is recommended to refrigerate the test samples and carry out the operations immediately after solving the environmental and operational problems,so as to ensure the accuracy of the final results and improve the efficiency of the test.
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