The value of two and a half pairs of hepatitis B(chemiluminescence method)combined with DNA test for the diagnosis of chronic hepatitis B and liver function assessment
Objective To analyze the value of two and a half pairs of hepatitis B(chemiluminescence method)combined with DNA test for the diagnosis of chronic hepatitis B and liver function assessment.Methods A total of 200 patients with chronic hepatitis B who underwent examination and treatment were selected as the subjects of this study,and were divided into an observation group(n=100)and a control group(n=100)according to different test methods.The patients in the observation group were received two and half pairs of hepatitis B(chemiluminescence method)combined with DNA test,and the patients in the control group received two and a half pairs of hepatitis B(chemiluminescence method)test.The positive rate of two and a half pairs of hepatitis B[hepatitis B core antibody(HBcAb),hepatitis B e antigen(HBeAg),Hepatitis B e antibody(HBeAb),hepatitis B surface antigen(HBsAg),hepatitis B surface antibody(HBsAb)]were compared.The correlation of HBsAg and HBeAg with hepatitis B virus DNA(HBV-DNA)and liver function indexes was analyzed,and the liver function indexes of HBeAg-positive patients and HBeAg-negative patients were compared.Results The positive rates of HBcAb,HBeAg,HBeAb,HBsAg,HBsAb in the observation group were 72.00%,56.00%,49.00%,69.00%,and 32.00%,which were significantly higher than 51.00%,41.00%,34.00%,55.00%,and 19.00%in the control group,and the differences were statistically significant(P<0.05).The percentage of HBV-DNA<103 copies/ml in HBsAg-positive patients was higher than that in HBsAg-negative patients,and the percentage of HBV-DNA 103-105 copies/ml was lower than that in HBsAg-negative patients,and the difference was statistically significant(P<0.05).There was no significant difference in the comparison of the percentage of alanine aminotrasferase(ALT)>40 U/L and aspartate transaminase(AST)>45 U/L between HBsAg-positive and HBsAg-negative patients(P>0.05).The percentage of HBV-DNA<103×copies/ml and the percentage of AST>45 U/L in HBeAg-positive patients were higher than those in HBeAg-negative patients,and the percentage of HBV-DNA>105 copies/ml was lower than that in HBeAg-negative patients,and the difference was statistically significant(P<0.05).There was no significant difference in the comparison of the percentage of ALT>40 U/L between HBsAg-positive and HBeAg-negative patients(P>0.05).In HBeAg-positive patients,the ALT was(70.24±3.52)U/L,the alkaline phosphatase(ALP)was(123.52±11.86)U/L,the direct bilirubin(DBil)was(10.68±1.57)μmol/L,the total bilirubin(TBil)was(23.49±2.39)μmol/L,the AST was(88.65±4.95)U/L,and the γ-glutamyltransferase(γ-GT)was(101.37±14.53)U/L,all of which were significantly higher than(59.37±2.16)U/L,(101.64±8.73)U/L,(7.83±0.85)μmol/L,(18.67±1.76)μmol/L,(62.76±3.27)U/L,and(86.72±7.64)U/L in the HBeAg-negative patients;the serum albumin(ALB)of(51.35±2.33)g/L was significantly lower than(57.96±3.41)g/L in HBeAg-negative patients;the difference was statistically significant(P<0.05).Conclusion Although the simple chemiluminescence method is simple and convenient for the detection of hepatitis B,the positive rates of hepatitis B virus markers are not as good as those of combined test of chemiluminescence method and DNA,and the higher diagnostic efficiency of combined test can effectively avoid the delay of misdiagnosis and missed diagnosis to patients,and the results of this test show that all liver function indexes of chronic hepatitis B patients are poor,so liver function indicators can also be used as the basis for judging the efficacy of clinical diagnosis of chronic hepatitis B and clinical treatment of chronic hepatitis B.
Chemiluminescence methodDNA testTwo and a half pairs of hepatitis B testChronic hepatitis B
万方数据
维普
