甲状腺功能生化免疫检验中化学发光免疫测定技术的应用价值分析

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中文摘要：目的探讨甲状腺功能生化免疫检验中化学发光免疫测定技术的应用价值。方法选择进行甲状腺功能生化免疫检验的 120 例疑似甲状腺功能障碍患者作为研究对象。采集患者的空腹外周静脉血液，分别采用放射免疫测定技术、化学发光免疫测定技术进行生化免疫检验。以临床综合诊断结果为依据，对比放射免疫测定技术、化学发光免疫测定技术对甲状腺功能障碍的诊断效能，分析两种方法的诊断结果与临床综合诊断结果之间的一致性。结果在 120 例疑似甲状腺功能障碍患者中，经临床综合诊断，确诊 31 例甲状腺功能亢进，其余 89 例患者无甲状腺功能亢进。化学发光免疫测定技术诊断甲状腺功能亢进的灵敏度、特异度、准确率、阳性预测值、阴性预测值分别为 96。77%、96。63%、96。67%、90。91%、98。85%，均比放射免疫测定技术的 77。42%、86。52%、84。17%、66。67%、91。67%更高(P<0。05)。经一致性分析，化学发光免疫检测结果与临床综合诊断结果之间保持高度一致，Kappa值=0。895;放射免疫检测结果与临床综合诊断结果之间仅为中度一致(Kappa值=0。631)。在 120 例疑似甲状腺功能障碍患者中，经临床综合诊断，确诊 65 例甲状腺功能减退，其余 55 例患者无甲状腺功能减退。化学发光免疫测定技术诊断甲状腺功能减退的灵敏度、特异度、准确率、阳性预测值、阴性预测值分别为 96。92%、98。18%、97。50%、98。44%、96。43%，均比放射免疫测定技术的 86。15%、85。45%、85。83%、87。50%、83。93%更高(P<0。05)。经一致性分析，化学发光免疫检测结果与临床综合诊断结果之间保持高度一致(Kappa值=0。876);放射免疫检测结果与临床综合诊断结果之间仅为中度一致(Kappa值=0。647)。结论在甲状腺功能生化免疫检验中，应用化学发光免疫测定技术能够对甲状腺功能亢进、甲状腺功能减退等甲状腺功能障碍进行灵敏、准确地诊断，其诊断价值比放射免疫测定技术更高。

外文标题：Application value analysis of chemiluminescence immunoassay in biochemical immunoassay of thyroid function

外文摘要：Objective To explore the application value of chemiluminescence immunoassay in biochemical immunoassay of thyroid function.Methods 120 suspected patients with thyroid dysfunction were selected for biochemical immunoassay of thyroid function.Fasting peripheral venous blood from patients were collected for biochemical immunoassay,radioimmunoassay and chemiluminescence immunoassay.Based on the clinical comprehensive diagnosis results,the diagnostic efficacy of radioimmunoassay and chemiluminescence immunoassay for thyroid dysfunction were compared,and the consistency between the two biochemical immunoassay and the clinical comprehensive diagnosis was analyzed.Results Among 120 patients with suspected thyroid dysfunction,31 cases of hyperthyroidism were diagnosed after comprehensive clinical diagnosis,and the remaining 89 patients did not have hyperthyroidism.The sensitivity,specificity,accuracy,positive predictive value,and negative predictive value of chemiluminescence immunoassay for the diagnosis of hyperthyroidism were 96.77%,96.63%,96.67%,90.91%,and 98.85%,which were higher than 77.42%,86.52%,84.17%,66.67%,and 91.67%of radioimmunoassay(P<0.05).After consistency analysis,the results of chemiluminescence immunoassay showed a high degree of consistency with the clinical comprehensive diagnosis(Kappa value=0.895);while the results of radioimmunoassay showed only moderate consistency with the clinical comprehensive diagnosis(Kappa value=0.631).Among 120 patients with suspected thyroid dysfunction,65 cases of hypothyroidism were diagnosed after comprehensive clinical diagnosis,and the remaining 55 patients did not have hypothyroidism.The sensitivity,specificity,accuracy,positive predictive value,and negative predictive value of chemiluminescence immunoassay for the diagnosis of hypothyroidism were 96.92%,98.18%,97.50%,98.44%and 96.43%,which were higher than 86.15%,85.45%,85.83%,87.50%and 83.93%of radioimmunoassay(P<0.05).After consistency analysis,the results of chemiluminescence immunoassay showed a high degree of consistency with the clinical comprehensive diagnosis(Kappa value=0.876);while the results of radioimmunoassay showed only moderate consistency with the clinical comprehensive diagnosis(Kappa value=0.647).Conclusion In the biochemical immunoassay of thyroid function,the application of chemiluminescence immunoassay can provide sensitive and accurate diagnosis of thyroid dysfunction such as hyperthyroidism and hypothyroidism,and its diagnostic value is higher than that of radioimmunoassay.

外文关键词：

Thyroid dysfunctionBiochemical immunoassayChemiluminescence immunoassayRadioimmunoassayHypothyroidismHyperthyroidism

作者：

贺勇锋、谢惠琴

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作者单位：

516300 惠东县人民医院检验科

关键词：

甲状腺功能障碍生化免疫检验化学发光免疫测定技术放射免疫测定技术甲状腺功能减退甲状腺功能亢进

出版年：

2024

DOI：