Objective To compare the influence of different blood collection methods on the results of clinical blood routine test.Methods A total of 80 outpatients receiving clinical blood routine test were selected,and their peripheral blood and venous blood were collected.Detecting and comparing the levels of blood routine indexes[white blood cell count(WBC),red blood cell count(RBC),platelet count(PLT),hemoglobin(HGB),hematocrit(HCT)],as well as the accuracy of blood routine tests in the two blood samples.Results WBC of(7.44±0.19)×109/L in the peripheral blood was lower than(8.59±0.23)×109/L in the venous blood;RBC,PLT,HCT and HGB were(5.30±0.25)×1012/L,(274.36±10.36)×109/L,(44.07±2.37)%and(143.96±8.74)g/L in the peripheral blood,which were significantly higher than(4.67±0.12)×1012/L,(261.10±9.85)×109/L,(41.28±1.35)%and(129.96±6.37)g/L in the venous blood(P<0.05).The accuracy of clinical blood routine test in the venous blood was 95.00%,which was significantly higher than 85.00%in the peripheral blood(P<0.05).Conclusion In blood routine test,there are obvious differences between peripheral blood collection and venous blood collection,with the accuracy of blood routine test being higher under the venous blood collection.