Study of centrifuge conditions for preparing rabbit leukocyte-poor platelet-rich plasma by single centrifugation
Objective To explore the best centrifuge condition for preparing rabbit leukocyte-poor platelet-rich plasma(LP-PRP)by using single centrifugation method.Methods Sixteen healthy New Zealand rabbits,aged 3-4 months,were utilized in the investigation.A total of 15 mL anticoagulated blood was extracted from the central ear artery of each rabbit,with a repeat of the blood collection procedure after 1 and 2 months.The obtained blood specimens were individually subjected to centrifugation at a radius of 16.7 cm and speeds of 1 200,1 300,1 400,and 1 500 r/min(equivalent to centrifugal forces of 269×g,315×g,365×g,and 420×g)for durations of 2,3,4,and 5 minutes,resulting in a total of 16 groups.Following centrifugation,collect plasma from each group to a distance of 1.5 mL from the separation plane.The volumes,platelet enrichment coefficient,and platelet recovery rates of LP-PRP in each group,under varying centrifugation conditions,were methodically computed and subsequently compared.Results The volume of LP-PRP obtained under all centrifugation conditions ranged from 1.8 to 7.6 mL.At a consistent centrifugal speed,an extension of centrifugation time leaded to a significant increase in the volume of LP-PRP,accompanied by a declining trend in the platelet enrichment coefficient of LP-PRP.When centrifuged for 2 minutes,the volume of LP-PRP at speeds of 1 200 and 1 300 r/min was less than 2.0 mL,while the volume of LP-PRP obtained under other conditions was more than 2.0 mL.When centrifuged for 4 and 5 minutes,the volume of LP-PRP obtained at each speed was more than 4 mL.LP-PRP with a platelet enrichment coefficient more than 2.0 could be prepared by centrifuging at 1 200 r/min for each time group and 1 300 r/min for 2 and 3 minutes,and the highest LP-PRP platelet enrichment coefficient could be obtained by centrifugation for 2 minutes at a speed of 1 200 r/min.The platelet recovery rates of LP-PRP obtained by centrifugation at 1 200 r/min for 4 and 5 minutes,as well as centrifugation at 1400 r/min for 5 minutes,were both greater than 60％.There was no significant difference between the groups when centrifuged at 1 200 r/min for 4 and 5 minutes(P＞0.05).Conclusion In the process of preparing rabbit LP-PRP using a single centrifugation method,collecting 15 mL of blood and centrifuging at a radius of 16.7 cm and speed of 1 200 r/min for 4 minutes can prepare LP-PRP with a volume exceeding 2.0 mL,platelet enrichment coefficient exceeding 2.0,and platelet recovery rate exceeding 60％.This centrifugal condition can achieve the optimal LP-PRP action parameters in the shortest possible time.
Single centrifugationleukocyte-poor platelet-rich plasmacentrifugal conditionrabbit
万方数据
维普
