In the present study,we investigated the effect of electrical fusion parameters on pig oocytes of enucleated zonafree by using different electrical field strength and the pulse duration and then observed cell fusion.The results showed that the comparison study of different electrical fusion parametes indicated that the rate of oocytes using one DC-pulse of 0.8 kV/cm for 30 μs (94.7％) groups were significantly higher than the other treatment groups(P＜0.05).The rate of fusion of the enucleated zona-free oocytes in one DC-pulse of 0.8 kV/cm for 30,60 and 90 μs group were 94.7％,86.5％ and 83.8％,respectively,showing that 30 μs treatment group were significant higher than those of 60 and 90 μs treatment groups(P＜0.05).The rate of fusion in one,double and three DC-pulse of 0.8 kV/cm for 30 μs were 94.7％,95.6％ and 81.7％,respectively,show-ing that double DC-pulse treatment groups were no significant higher than those of one DC-pulse treatment groups (P＞0.05),but both were significant higher than three DC-pulse treatment groups,the fusion rate of them was significant higher than three DC-pulse treatment groups(P＜0.05).
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万方数据
维普
