Establishment and Application of Method for PCR Detection of Beef Ingredient in Meat Products
This study established a PCR method for detection the beef ingredient in meat products according to the bovine specific mitochondrial DNA fragment,and 67 meat products of cow were detected using PCR.The results showed that a specific amplification fragment of the expected size was detected and demonstrated,buffalo,yak,cow and bovine meat were positive using this method,whereas the 14 kinds of animal meat such as sheep,equine,canis,donkey,rabbit and duck were negative,the detection limit was 53.2 fg/μL DNA.The positive rate of 67 meat products of cow was 100％.The results indicated that the method was quickness,convenience,sensitivity and specificity,and can be used for the identification of bovine source of beef products components.
国家科技期刊平台
NSTL
万方数据
维普
