Comparative Analysis of Diversity of Milk Microbiota in Cows with Mastitis and Healthy Cows
[Objective]In order to further understand the relationship between mammary microecological changes and the etiology of mastitis,the diversity and composition of microflora in milk of healthy cows,cows with subclinical mastitis and cows with clinical mastitis were compared in this study.[Method]Firstly,PCR was used to detect the main mastitis-related pathogens in the milk of healthy cows,subclinical mastitis and clinical mastitis.Furthermore,16S rRNA high-throughput sequencing technology was applied to analyze the microbial diversity,microbiota composition and predictive function of milk from healthy cows,cows with subclinical mastitis and clinical mastitis,respectively.[Result]Compared with the milk of healthy cows,the composition of milk microbiota in cows with subclinical mastitis and clinical mastitis had significant changes at phylum and genus levels;The abundances of Staphylococcus,Rolstonia,Brevibacterium,Ligilactobacillus and Blautia were significantly increased(P＜0.05)or extremely significantly increased(P＜0.01),and Rolstonia and Ralstonia pickettii were significantly enriched(P＜0.05)in the milk of subclinical mastitis;The relative abundances of Lactobacillus,Staphylococcus and Limosilactobacillus were significantly increased(P＜0.05)in milk of cows with clinical mastitis,and Bacteroidetes,Bacillus,Staphylococcus and Staphylococcus caprae were significantly enriched(P＜0.05).The abundances of Bacteroides,Treponema and Streptococcus in milk of cows with clinical mastitis were significantly higher than that of milk with subclinical mastitis(P＜0.05).Pseudomonas and Azospira were biomarkers of healthy milk,Rolstonia and Ralstonia pickettii were biomarkers of subclinical mastitis milk,and Staphylococcus and Staphylococcus caprae were biomarkers of clinical mastitis milk.Compared with the milk of healthy cows,the polysaccharide biosynthesis and metabolic functions of microorganisms in milk of subclinical mastitis were extremely significantly down-regulated(P＜0.01),while the carbohydrate metabolism,folding classification and degradation,replication and repair,energy metabolism,cofactor and vitamin metabolism,translation,nucleotide metabolism,cell growth and death of microorganisms in milk of clinical mastitis were extremely significantly up-regulated(P＜0.05).The functions of cell movement,metabolism,signal transduction,neurodegenerative diseases,nervous system and other functions were extremely significantly down-regulated(P＜0.01),and the functions of signal molecules and interactions were significantly down-regulated(P＜0.05).[Conclusion]The alteration of microflora diversity in the milk of cows with subclinical mastitis and clinical mastitis were initially characterized in this study.The results showed that the diversity,species composition and function of milk microflora of cows with subclinical mastitis and clinical mastitis were significantly changed.This might be closely related to the cause of cow mastitis and the degree of mammary inflammation.
国家科技期刊平台
NSTL
万方数据
维普
