首页|鸡球虫体外培养模型及应用研究进展

鸡球虫体外培养模型及应用研究进展

扫码查看
原文链接
  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
  • 维普
鸡球虫病是由艾美耳球虫寄生于鸡肠上皮细胞内所引起的一种寄生性原虫病,感染鸡体重减轻、营养不良、肠道损伤等,严重危害养鸡业的健康发展.球虫的生命周期包括从无性阶段到有性阶段的转换并局限于一个宿主.目前对球虫的研究主要包括在细胞生物学及其不同生命阶段的蛋白质表达和运输机制、宿主细胞入侵和宿主-寄生虫相互作用、新型药物靶标筛选等方面.鉴于研究问题的多样性,以及减少和取代动物试验的要求,建立高效的体外培养模型成为研究鸡球虫生物学特性、抗球虫疫苗和药物的基本条件.笔者重点阐述了鸡球虫体外培养模型及其在药物抗虫效果研究中的应用,包括鸡胚培养模型、二维培养模型(原代细胞培养模型、传代细胞系培养模型)及三维类器官培养模型.越来越多培养技术的突破为研究球虫生命周期阶段和干预策略开辟了新的途径,也为深入了解鸡球虫致病机制、抗球虫药物研发提供依据.
Research Progress on Chicken Coccidia in vitro Culture Models and Application
Coccidiosis is a parasitic protozoan disease caused by one or more species of Eimeria parasitizing in the intestinal epithelial cells of chickens.The disease quickly leads to weight loss,malnutrition,intestinal damage,etc.,and has a high economic impact on the poultry industry worldwide.The life cycle of coccidia includes a switch from asexual to sexual stages and is often restricted to a single host.Current research mainly focused on cell biology,protein expression and transport mechanisms in different life stages,host cell infection,host-parasite interactions,new anticoccidial drug screens,etc.Given the diversity of research questions and the requirement of reduce and replace animal experiments,establishing efficient in vitro culture models has become essential for coccidia biological characteristics,anti-coccidia vaccines and drugs study.In this review,the author summarized the in vitro culture model of the poultry parasite coccidia and its application in drug screening,including the chicken embryo culture model,two-dimensional culture models(primary cell culture model,immortalized cell line culture model)and 3D organoid culture model.Breakthroughs in culture techniques have opened new avenues for research on the life cycle stages of coccidia and new intervention strategies and also provide a reference for in-depth understanding of the pathogenic mechanism of coccidia and the development of the anti-coccidia drugs.

chickenEimeriain vitro culture model

潘晓婷、李娟、陈兵、刘俊杰、戚南山、廖申权、吕敏娜、林栩慧、蔡海明、胡俊菁、宋勇乐、白银山、孙铭飞

展开 >

广东省农业科学院动物卫生研究所,广东省畜禽疫病防治研究重点实验室,农业农村部禽流感等家禽重大疾病防控重点实验室,广州 510640

佛山科学技术学院,佛山 528000

深圳海关动植物检验检疫技术中心,深圳 518038

鸡 艾美耳球虫 体外培养模型

广东省农科院协同创新中心项目广东省科技计划猪禽种业全国重点实验室开放基金科技创新战略专项(高水平农科院建设)科技创新战略专项(高水平农科院建设)科技创新战略专项(高水平农科院建设)科技创新战略专项(高水平农科院建设)科技创新战略专项(高水平农科院建设)科技创新战略专项(高水平农科院建设)科技创新战略专项(高水平农科院建设)科技创新战略专项(高水平农科院建设)

XTXM2022022021B12120500212022GZ07202110TD202122TDR2020PY-JC001R2019YJ-YB3010R2020PY-JG013R2020QD-048R2021PY-QY007R2023PY-JG018

2024

10.16431/j.cnki.1671-7236.2024.01.029

中国畜牧兽医
中国农业科学院北京畜牧兽医研究所

中国畜牧兽医

CSTPCD北大核心
影响因子：0.72
ISSN：1671-7236
年,卷(期)：2024.51(1)
  • 11