Alleviative Effects of Three Traditional Chinese Medicine Components on Liver Injury in Zearalenone Poisoned Mice
[Objective]This study was aimed to explore the relieving effect of traditional Chinese medicine on liver damage caused by zearalenone(ZEA)in animals.[Method]63 8-week-old Kunming female mice were randomly divided into 7 groups,with 9 mice in each group.In control group,physiological saline was administered orally once in the morning and once in the afternoon;ZEA group,20 mg/kg ZEA was gavaged in the morning and physiological saline was gavaged in the afternoon.Quercetin,kaempferol,rutin,and compound ingredients groups were traditional Chinese medicine treatment groups.In the morning,20 mg/kg ZEA was administered by gavage,and in the afternoon,50 mg/kg quercetin,50 mg/kg kaempferol,100 mg/kg rutin,and 100 mg/kg compound ingredients(V(quercetin)∶V(kaempferol)∶V(rutin)=1∶2∶2)were administered by gavage,respectively.Positive control group,20 mg/kg ZEA were administered by gavage in the morning and 100 mg/kg vitamin E was administered by gavage in the afternoon.Each animal was gavaged with 0.2 mL each time for 7 consecutive days.The mice were euthanized on the 8th day to detect body weight,liver index,serum liver function,antioxidant enzyme activity,and liver inflammation indicators.[Result]The weight development results showed that compared with control group,ZEA group mice had lower body weight and higher liver weight(P＜0.05).Compared with ZEA group,the weight of rutin and compound groups were increased(P＜0.05).Compared with control group,the serum liver function test results showed that the activities of alanine aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALP),acid phosphatase(ACP),lactate dehydrogenase(LDH)in ZEA group were increased(P＜0.05).Compared with ZEA group,the activity of various enzymes in traditional Chinese medicine and vitamin E treatment groups were decreased(P＜0.05).The results of liver oxidative stress test showed that compared with control group,the activity of superoxide dismutase(SOD),catalase(CAT),glutathione(GSH)content,and total antioxidant capacity(T-AOC)in liver of ZEA group were decreased(P＜0.05),while the content of malondialdehyde(MDA)and LDH were increased(P＜0.05).Compared with ZEA group,the activity of SOD,CAT,GSH content and T-AOC in traditional Chinese medicine and vitamin E treatment groups were increased(P＜0.05),while the content of MDA and LDH were decreased(P＜0.05).Inflammatory factor detection results showed that compared with control group,the content of tumor necrosis factor-α(TNF-α),interleukin-1 β(IL-1β)and IL-6 in ZEA group were increased and the content of IL-10 was decreased(P＜0.05).Compared with ZEA group,TNF-α,IL-1β and IL-6 contents of traditional Chinese medicine ingredient and vitamin E treatment groups were decreased(P＜0.05),while the IL-10 content were increased in quercetin and vitamin E treatment groups(P＜0.05).[Conclusion]All three traditional Chinese medicine ingredients quercetin,kaempferol,rutin and vitamin E could alleviate the liver damage caused by ZEA in mice,and the effect of kaempferol was the best.
国家科技期刊平台
NSTL
万方数据
维普
