[Objective]This study was aimed to investigate the effect of myelocytomatosis viral oncogene homolog(c-MYC)on cat fibroblasts and its relationship with cadherin 1(CDH1)gene expression and molecular characteristics,so as to provide a basis for application in the prevention and treatment of tumor diseases and the repair of tissue damage in cat.[Method]Cat fetal fibroblasts were isolated and cultured by tissue adherence,and the PB-TRE-c-MYC plasmid was transfected into the cells by electroporator,then the cell morphology was observed.The expression of CDH1 gene was detected by Real-time quantitative PCR,and the physicochemical properties and structural characteristics of CDH1 protein were analyzed by bioinformatics software.[Result]c-MYC overexpression led to the changes in cell morphology,with a transition from the long shuttle shape of mesenchymal cells to the pebble shape of epithelial cells,and the expression of epithelial cell marker gene CDH1 was extremely significantly upregulated(P＜0.01).Bioinformatics analysis results showed that CDH1 protein in cat had 881 amino acids,of which the most abundant was leucine,localized in cell membrane,with 4 CA domains,which mediated cell-cell contact.CDH1 protein belonged to hydrophilic acidic proteins,mainly composed of random coil,and might have signaling peptide sites transported by Sec translocons and cleaved by signal peptidase Ⅰ(Sec/SP Ⅰ).[Conclusion]The expression of CDH1 gene in cat could be activated by exogenous reprogramming factor c-MYC,and its encoding protein cadherin could promote the mesenchymal-to-epithelial transition of cat fetal fibroblasts to inhibit cell carcinogenesis.
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