Study on the Co-regulation of m6A and miRNA on Skeletal Muscle Development of Peking Ducks in Embryonic Stage
[Objective]The aim of this experiment was to study the regulation of miRNA and N6-methyladenosine(m6A)in the development of breast muscle of Peking ducks at embryonic stage,in order to lay a foundation for understanding the development of breast muscle of Peking ducks.[Method]The breast muscles of duck embryo at E13 and E27 stages were collected.Methylated RNA immunoprecipitation with next generation sequencing(MeRIP-Seq)and microRNA sequcencing(miRNA-Seq)were used to identify potential differential genes regulated by m6 A in the differentiation process of breast muscle cells in Beijing duck embryos,and their functions were analyzed in combination with miRNA and its target genes.[Result]The miRNA-Seq results showed that there were 181 significantly different miRNAs,and a total of 11 176 targets were predicted through target gene prediction,including m6A related genes and muscle development related genes.14 344 and 14 016 m6A peaks were detected by MeRIP-Seq in E13 and E27 groups,respectively,mapping to 8 248 known genes and 7 763 known genes.A total of 3 240 m6A were mapped to 2 767 differentially methylated genes(DMGs)during E13 and E27 stages.GO and KEGG analysis showed that DMGs were mainly enriched in pathways related to muscle development and fat deposition,as well as many genes related to muscle development,such as MTPN,MYF6 and MYF5 genes.556 differentially expressed genes(DEGs)were detected by miRNA-Seq.GO and KEGG pathway analysis results indicated that these DEGs were significantly enriched in the Wnt signaling pathway,metabolic pathway,and fatty acid,suggesting that DEGs might be involved in adipocyte differentiation and fat metabolism processes.The association analysis between miRNA-Seq and m6A-Seq showed that a total of 1 517 differential expression and methylation genes(DEMGs)were detected.GO and KEGG analysis of these DEMGs showed that DEMGs were mainly enriched in muscle development related pathways.[Conclusion]m6 A methylation modification and miRNA had important effects on skeletal muscle development and fat deposition in Peking ducks.
miRNAm6 A methylation modificationhigh throughput sequencingPeking duck
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