Effect of Feeding Zinc Lysine on Plasma Biochemical Indices and Sperm Quality of Hu Sheep Rams in Autumn in Northeastern China
[Objective]This experiment was aimed to investigate the effects of feeding zinc lysine on plasma biochemical indices and sperm quality of Hu sheep in autumn in Northeastern China.[Method]Thirty-two 1-2 years old Hu sheep rams with well body condition were randomly divided into four groups fed with 0,25,50 and 100 mg/d zinc lysine,respectively.The experiment was conducted in Yichun,Heilongjiang from August to October 2022,with an average temperature of 10.3 ℃.The transition period was 7 days,and the experimental period was 56 days.On the 28th,42nd and 56th days,the jugular vein blood of Hu sheep was collected to determine plasma testosterone,melatonin,interleukin-2,interleukin-4,interleukin-6,interleukin-10,adrenocorticotropic hormone,and tumor necrosis factor-α contents,as well as total antioxidant capacity,catalase,superoxide dismutase,glutathione peroxidase activities and malondialdehyde content.Hu sheep semen was collected to determine sperm viability,plasma membrane integrity,and abnormality rate.[Result]On the 28th day,the melatonin content of the 50 mg/d group was significantly higher than that of the 0 and 100 mg/d groups(P＜0.05),the total antioxidant capacity was significantly higher than that of the 0 mg/d group(P＜0.05),the interleukin-4 content was significantly lower than that of the 25 mg/d group(P＜0.05),and the interleukin-10 content was significantly lower than that of the 0 mg/d group(P＜0.05).On the 42nd day,the superoxide dismutase activity of the 50 mg/d group was significantly higher than that of the 0 mg/d group(P＜0.05),the interleukin-4 content was significantly lower than that of the other groups(P＜0.05),and the adrenocorticotropic hormone content was significantly lower than that of the 0 and 100 mg/d groups(P＜0.05).On the 56th day,the sperm plasma membrane integrity rate of the 50 mg/d group was significantly higher than that of the 0 mg/d group(P＜0.05),the superoxide dismutase activity was significantly higher than that of the 0 mg/d group(P＜0.05),and the interleukin-4 content was significantly lower than that of the 0 and 100 mg/d groups(P＜0.05).[Conclusion]Supplementing with zinc lysine could ameliorate the plasma reproductive hormones content,antioxidant capacity,immune indexes content and sperm quality of Hu sheep rams in autumn in Northeastern China.The additive amount was recommended 50 mg/d.
万方数据
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