Effects of Conditioning Training at Different Stages on Fecal Metabolomics in Yili Horses
[Objective]This study was aimed to explore the change characteristics of fecal metabolites in Yili horses at different stages of conditioning training,and initially screen the differential metabolites affected by conditioning training in Yili horses.[Method]Ten galloping Yili horses with consistent management and untrained training were selected from Zhaosu horse farm in Yili,Xinjiang,and divided into two groups,one group for special training,and the other group for untrained.Feces samples were collected on the 30th and 60th day of training,non-targeted metabolome detection was performed by LC-MS method,and the detection results were analyzed.[Result]Compared with training(QY)and untraining(QB)groups at the 30th day,there were significant differences in 27 positive ion mode metabolites and 20 negative ion mode metabolites,and metabolites such as 2-arachidyl glycerol,propionyl L-carnitine and creatine were significantly upregulated.The metabolites were mainly concentrated in β-alanine metabolism,arginine and proline metabolism,pentose phosphate pathway,arachidonic acid metabolism,5-hydroxytryptaminergic synapse and other metabolic pathways.There were 57 positive ion mode metabolites and 33 negative ion mode metabolites between training(HY)and untraining(HB)groups at the 60th day.The metabolites of palmitoleic acid,palmitic acid,methyltestosterone and melatonin were significantly upregulated,while the metabolites of cortisol and folate were significantly downregulated.The metabolites were mainly concentrated in steroid hormone biosynthesis,Cushing syndrome,cortisol synthesis and secretion,neuroactive ligand-receptor interaction,fatty acid biosynthesis,unsaturated fatty acid biosynthesis and other metabolic pathways.[Conclusion]There were differences in fecal metabolites of Yili horses at different stages of conditioning training,which provided theoretical basis for improving the overall health level of horses and promoting exercise performance.
Yili horseconditioning trainingmetabolitemetabolic pathway
万方数据
维普
