Effects of Cardamomine on Organ Coefficients,Serum Biochemical Indexes,Antioxidant Capacity and Inflammatory Factors of Chronically Heat-stressed Danzhou Chickens
[Objective]The study aimed to explore the effects of cardamomamine(CDN)on organ coefficients,serum biochemical indexes,antioxidant capacity and inflammatory factors in chronic heat-stressed Danzhou chickens.[Method]200 1-day-old Danzhou hens were selected for the experiment and randomly divided into 5 groups:Control group(CON),heat stress group(HS),low-dose(L-CDN),medium-dose(M-CDN),and high-dose cardamom group(H-CDN).Each group had 4 replicates,with 10 chickens per replicate.The hens in CON and HS groups were fed a basic diet,while in L-CDN,M-CDN,and H-CDN groups were added 50,100,and 200 mg/kg cardamom to the basic diet,respectively.The experiment lasted for 42 days,with a pre-trial period of 21 days and a formal trial period of 21 days.During the formal trial period,the HS,L-CDN,M-CDN,and H-CDN groups were kept at a temperature of(36±2)℃ from 09:00 to 16:00 each day.The rest of the time,the temperature was maintained at(25±2)℃,while the CON group was kept at a temperature of(25±2)℃ all day.At the end of 42 days,two Danzhou chickens with similar weights were selected from each replicate for slaughter.Serum was collected to detect biochemical indicators,antioxidant indicators,and serum inflammatory factors.The liver,spleen,thymus,and bursa of Fabricius were collected to calculate organ coefficients.[Result]① The levels of alanine aminotransferase(ALT)in the serum of Danzhou chickens in M-CDN and H-CDN groups were significantly lower than those in HS group(P＜0.05),while the levels of aspartate aminotransferase(AST),lactate dehydrogenase(LDH),total cholesterol(TC),and triglycerides(TG)in the serum of chickens in L-CDN,M-CDN,and H-CDN groups were significantly lower than those in HS group(P＜0.05).Except for the significantly lower TG content in H-CDN group compared to CON group(P＜0.05),there was no significant difference between H-CDN group and CON group(P＞0.05).② Compared with HS group,the Fabricius index of Danzhou chickens in M-CDN and H-CDN groups were significant increased(P＜0.05),while the spleen index in H-CDN group was significant increased(P＜0.05).The liver index of all three experimental groups were decreased significantly(P＜0.05),and there was no significant difference between them and CON group(P＞0.05).Among them,the Fabricius index of M-CDN and H-CDN groups was significantly higher than that of L-CDN group(P＜0.05),and the spleen index of H-CDN group was significantly higher than that of L-CDN and H-CDN groups(P＜0.05).③ Compared with HS group,the serum malondialdehyde(MDA)content of Danzhou chickens in the three experimental groups was significantly reduced(P＜0.05).The serum superoxide dismutase(SOD)activity of Danzhou chickens in M-CDN group and the serum SOD,catalase(CAT),and glutathione peroxidase(GSH-Px)activities of Danzhou chickens in H-CDN group were significantly increased(P＜0.05),and there was no significant difference compared with CON group(P＞0.05).The total antioxidant capacity(T-AOC)of the serum of Danzhou chickens in three experimental groups was significantly increased(P＜0.05),but still significantly lower than that of CON group(P＜0.05).The SOD activity in M-CDN and H-CDN groups was significantly higher than that in L-CDN group(P＜0.05),while the CAT and GSH-Px activities in H-CDN group were significantly higher than those in L-CDN and M-CDN groups(P＜0.05).④ Compared with HS group,the serum interleukin-1β(IL-1β)content of Danzhou chickens in all three experimental groups were significantly reduced(P＜0.05).The serum IL-6 and tumor necrosis factor-α(TNF-α)contents of Danzhou chickens in M-CDN and H-CDN groups were significantly reduced,while the IL-10 levels were significantly increased(P＜0.05),and there was no significant difference compared to CON group(P＞0.05).There was no significant difference between the three experimental groups(P＞0.05).[Conclusion]Cardamom added to feed could regulate some serum biochemical indexes of Danzhou chickens under heat stress,promote the development of immune organs to enhance immune ability,alleviate heat stress-induced liver injury,improve antioxidant ability,and alleviate heat stress-induced immune stress and inflammation.The appropriate supplement level of cardamom for chronic heat stress in Danzhou chickens was 200 mg/kg.
万方数据
维普
