Polymorphisms of LPL and NUDT3 Genes and Its Association Analysis with Meat Quality Traits in Beijing Black Pigs
[Objective]This study was aimed to investigate the association between the polymorphism of lipoprotein lipase(LPL)and Nudix hydrolase 3(NUDT3)genes with intramuscular fat(IMF)content and backfat thickness in Beijing Black pigs,in order to guide the breeding of Beijing Black pigs at the molecular level.[Method]Using 347 samples of the longissimus dorsi muscle in Beijing Black pigs,the IMF content and back fat thickness data were measured,and the high and low IMF content was grouped.Primers were designed based on NUDT3 and LPL gene sequences of pigs,and PCR amplification and sequencing were performed.The sequencing results were analyzed using SeqMan in DNAStar softwares.Duncan's multiple test statistical analysis was used to analyze the association between different genotypes of NUDT3 and LPL genes and IMF content and backfat thickness in Beijing Black pigs.The gene expression difference was analyzed using Real-time quantitative PCR.[Result]There were 1 missense mutation,3 synonymous mutations and 3 3'-UTR mutations in LPL gene,and 6 3'-UTR mutations in NUDT3 gene.The c.431 G＞C of LPL gene CDS was significantly associated with 6-7 rib back fat thickness,c.647 G＞C was significantly associated with thoracolumbar junction back fat thickness,and c.637 A＞G was significantly correlated with 6-7 rib back fat thickness,at the thoracolumbar junction back fat thickness and lumbar sacral junction back fat thickness in Beijing Black pigs(P＜0.05).The c.1683 A＞G of LPL gene 3'-UTR region was significantly correlated with backfat thickness at the thoracolumbar junction;c.1689 C＞A was significantly correlated with IMF content in Beijing Black pigs(P＜0.05).The mutation sites of NUDT3 gene all occured in 3'-UTR region,c.7204 G＞T was significantly correlated with IMF content and back fat thickness at the lumbar sacral junction,c.6068 C＞G was significantly correlated with IMF content and 6-7 rib back fat thickness,c.5729 G＞A was significantly correlated with IMF content in Beijing Black pigs(P＜0.05).Real-time quantitative PCR analysis showed that there was no significant difference in the expression of NUDT3 gene c.7204 G＞T between the high and low IMF content groups,and there was also no significant difference in the expression of LPL gene c.1689 C＞A between CC and AA genotypes(P＞0.05).[Conclusion]The mutation sites of LPL and NUDT3 genes were significantly associated with IMF content in Beijing Black pigs,providing new molecular markers for meat quality breeding in Beijing Black pigs.
Beijing Black pigsNUDT3 geneLPL genemeat qualitycorrelation analysis
万方数据
维普
