首页|柯乐猪MMP2基因多态性及其与繁殖性状的关联分析

柯乐猪MMP2基因多态性及其与繁殖性状的关联分析

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[目的]探究基质金属蛋白酶2(matrix metallopeptidase 2,MMP2)基因单核苷酸多态性(single nucleotide polymorphisms,SNP)及其与繁殖性状的相关性,筛选与柯乐猪繁殖性状相关的遗传标记.[方法]试验选择212头健康的经产柯乐母猪,采用直接测序法筛选柯乐猪MMP2基因SNP位点,利用RNAfold在线软件预测MMP2基因不同单倍型mRNA二级结构,并通过SPSS 22.0软件分析MMP2基因SNP位点与柯乐猪繁殖性状的相关性.[结果]在柯乐猪MMP2基因第2外显子中检测到1个SNP位点(g.30062403 C＞T),在第4外显子中检测到2个SNPs位点(g.30065309 C＞T和g.30065312 C＞T),均存在3种基因型(CC、CT和CT).遗传特性分析结果显示,g.30062403 C＞T位点为低度多态,g.30065309 C＞T和g.30065312 C＞T位点属于中度多态.卡方适合性检验结果显示,g.30062403 C＞T位点显著偏离Hardy-Weinberg平衡(P＜0.05),另2个位点均符合Hardy-Weinberg 平衡(P＞0.05).连锁不平衡分析结果显示,g.30065309 C＞T和g.30065312 C＞T位点之间存在强连锁不平衡.单倍型mRNA二级结构分析显示,单倍型H1的mRNA二级结构与原序列相同,其他3种单倍型均导致mRNA二级结构和最小自由能发生变化.关联分析结果显示,g.30062403 C＞T位点TT基因型个体的窝产活仔数显著高于CC基因型,断奶窝重显著高于CT基因型(P＜0.05);g.30065309 C＞T位点CC基因型个体的断奶仔数显著高于TT基因型(P＜0.05);g.30065312 C＞T位点TT基因型个体的窝产活仔数、断奶仔数均显著高于CT基因型(P＜0.05).双倍型H2H2为窝产仔数、窝产活仔数、断奶仔数、断奶窝重的优势双倍型,H1H4为初生窝重的优势双倍型.[结论]柯乐猪MMP2基因3个SNPs位点与其窝产仔数、窝产活仔数、初生窝重、断奶仔数及断奶窝重均存在显著关联性,可作为分子育种遗传标记.
Polymorphism of MMP2 Gene and Its Association with Reproductive Traits in Kele Pigs
[Objective]The experiment was conducted to explore single nucleotide polymorphisms(SNP)of matrix metallopeptidase 2(MMP2)gene and its correlation with reproductive traits,and to screen genetic markers related to reproductive traits of Kele pigs.[Method]A total of 212 healthy partriparous Kele sows were selected and the SNP of MMP2 gene were screened by direct sequencing.Using RNAfold online software to predict the secondary mRNA structure of different haplotypes of MMP2 gene.SPSS 22.0 software was used to analyze the correlation between MMP2 gene SNP and reproductive traits of Kele pigs.[Result]1 SNP(g.30062403 C＞T)was detected in exon 2 and 2 SNPs(g.30065309 C＞T and g.30065312 C＞T)were detected in exon 4 of MMP2 gene,all with 3 genotypes(CC,CT and CT).The results of genetic analysis showed that g.30062403 C＞T was a low polymorphism,and g.30065309 C＞T and g.30065312 C＞T were moderate polymorphisms.Chi-square fitness test results showed that g.30062403 C＞T significantly deviated from Hardy-Weinberg equilibrium(P＜0.05),and the other 2 SNPs were in Hardy-Weinberg equilibrium(P＞0.05).Linkage disequilibrium analysis showed that there was strong linkage disequilibrium between g.30065309 C＞T and g.30065312 C＞T.The analysis of haplotype mRNA secondary structure showed that the mRNA secondary structure of haplotype Hl was the same as the original sequence,and the other 3 haplotypes all caused changes in the mRNA secondary structure and minimum free energy.The results of association analysis showed that the litter number of TT genotype at g.30062403 C＞T was significantly higher than that of CC genotype,and the weaning litter weight was significantly higher than that of CT genotype(P＜0.05).The number of weaned piglets of CC genotype at g.30065309 C＞T was significantly higher than that of TT genotype(P＜0.05).The number of live litter and weaned piglets of TT genotype at g.30065312 C＞T was significantly higher than that of CT genotype(P＜0.05).The diplotype H2H2 was the dominant diplotype of litter number,litter born alive,weaned piglets and weaning litter weight,while H1 H4 was the dominant diplotype of litter weight at birth.[Conclusion]There was a significant correlation between of MMP2 gene 3 SNPs and litter number,litter born alive,litter weight at birth,weaned piglets and litter weight at weaning,which could be used as genetic markers for molecular breeding.

Kele pigsMMP2 genepolymorphismreproductive traitsassociation analysis

向进、杨齐心、罗华伦、洪亚婷、郭小江、杨红文、李维、王春源、吴燕、张依裕

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贵州大学动物科学学院,高原山地动物遗传育种与繁殖教育部重点实验室,贵州省动物遗传育种与繁殖重点实验室,贵州大学香猪研究所,贵阳 550025

贵州省畜禽遗传资源管理站,贵阳 550001

黔西南州农业农村局,兴义 562400

贵州省草地技术试验推广站,贵阳 550001

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柯乐猪 MMP2基因 多态性 繁殖性状 关联分析

贵州省科技支撑计划贵州省科技计划贵州省生猪产业发展项目贵州省生猪产业发展项目贵州省种业发展项目贵州省生猪产业技术体系建设项目国家重点研发计划子课题

黔科合支撑[2021]一般147黔科合平台人才[2021]5630号黔财农[2021]157号黔财农[2022]183号黔农计财[2022]10号GZSZCYJSTX-032022YFD1100308-01

2024

10.16431/j.cnki.1671-7236.2024.03.026

中国畜牧兽医
中国农业科学院北京畜牧兽医研究所

中国畜牧兽医

CSTPCD北大核心
影响因子：0.72
ISSN：1671-7236
年,卷(期)：2024.51(3)
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