Research Progress on in vitro Maturation of Small Follicle-derived Oocytes in Mammalian
In vitro maturation is the main way for mammalian embryo engineering to obtain mature oocytes.The quality of oocytes directly affects early embryonic development,establishment and maintenance of pregnancy,and fetal development.The large number of small follicles on the surface of mammalian ovaries can provide oocytes that apply to embryo engineering,but the oocytes obtain from the ovary are at different stages that shows different mature capacity,and the quality of in vitro maturation of oocytes from small follicles are poor and deficient in developmental ability.How to improve the in vitro maturation ability of small follicle oocytes to fully tap into the genetic resources of excellent female animals and improve the efficiency of embryo engineering has become a research hotspot.Based on this,the review summarizes the research progress of in vitro maturation of small follicle oocytes in mammalian,compares the differences in accumulation of oocyte material,the differences in the composition and content of follicular fluid,and the differences between follicular granulosa cells and cumulus cells in large and small follicles,analyzes the reasons for the poor quality of mammalian small follicle oocytes in vitro maturation,analyzes and summarizes the measures to improve the developmental ability of mammalian small follicle oocytes in vitro maturation,including inhibition of nuclear maturation,pre-maturation culture of oocyte and co-culture of oocytes with granulosa and cumulus cells,which aimes at providing theoretical guidance for the research of the development regulation mechanism of small follicle oocytes and the improvement of developmental capacity.
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维普
