[Objective]This study was aimed to screen the traditional Chinese medicine(TCM)against avian Infectious bronchitis virus(IBV)and verify therapeutic effect,so as to provide a reference for clinical medication.[Method]The chicken embryo culture method was used to study the anti-IBV screening test which selected 24 single TCM and 6 commercially available compound TCM.The experimental procedure was to inject TCM in SPF chicken embryos first and then inoculate the virus 2 h later or inoculate the virus first and then inject TCM 2 h later.The variance(ANOVA)analysis of chicken embryo weight was performed with SPSS 20.0 software,and Real-time quantitative PCR was used to assist in determining the therapeutic effect.[Result]ANOVA on the net weight of chicken embryos showed that there was significant difference between Platycodon grandi florus(Jacq.)A.DC and positive control group(P＜0.05),and there was no significant difference with negative control group(P＞0.05),indicating that Platycodon grandiflorus(Jacq.)A.DC had preventive and curative effects.The viral load in allantoic fluid by Real-time quantitative PCR showed that Platycodon grandiflorus(Jacq.)A.DC,Houttuynia cordata Thunb,Xuanfeibaidu prescription,Gynostemma pentaphyllum(Thunb.)Makino,Taraxacum mongolicum Hand.-Mazz and Glycyrrhiza uralensis Fisch could inhibit the proliferation of IBV,and the inhibitory effect of Platycodon grandiflorus(Jacq.)A.DC was the best.[Conclusion]Platycodon grandiflorus(Jacq.)A.DC had the remarkably effective for treating IBV,which laid a reference for the in-depth study of the mechanism of Platycodon grandiflorus(Jacq.)A.DC.
关键词
鸡传染性支气管炎病毒(IBV)/鸡胚/中药/筛选
Key words
avian Infectious bronchitis virus(IBV)/chicken embryo/traditional Chinese medicine/screening
维普
万方数据