Study on the Application of Propagation of Superfine-type Erlangshan Cashmere Goat by Somatic Cell Cloning Technology
[Objective]The cloned individual of superfine-type Erlangshan cashmere goat was produced by somatic cell cloning technology,which provided a theoretical basis for the application of this technology in the expansion of local high-quality sheep breed resources and the breeding of new varieties.[Method]A 4-year old Erlangshan cashmere goat with a cashmere fineness of 13.89 µm was selected.The ear tissue was collected and cultured as fibroblasts,which were used as the donor cells.Somatic cell cloning technology was used to expand the propagation of superfine-type Erlangshan cashmere goat.The feasibility of the technique was evaluated by weight,fineness of cashmere and reproductive performance of cloned sheep.[Result]The stable system of goat oocyte maturation culture in vitro and somatic cell cloning was successfully established.The oocyte maturation rate was 44.8％,and the cleavage rate and blastocyst rate of parthenogenesis was 83.0％and 22.4％,respectively.The cleavage rate and blastocyst rate of the cloned embryos of Erlangshan cashmere goat was 77.8％and 13.7％,respectively.5 cloned sheep were obtained with normal growth and development,and 16 healthy F1 generation individuals were obtained by natural mating with Erlangshan ewes,with normal reproductive ability.The cashmere fineness of cloned individuals was significantly lower than that of control individuals of the same age(P＜0.01).[Conclusion]Superfine-type Erlangshan cashmere goat individuals was successfully cloned by somatic cell cloning technology,which had normal production and reproductive performance,and retained the characteristics of superfine cashmere,laid a foundation for the expansion of native excellent sheep germplasm resources and the application of new varieties breeding.
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