Study on the Quality and Microbial Diversity of Mixed Silage Between Distillers Grains and Navel Orange Residue
[Objective]This experiment was to explore the optimal ratio of the mixed silage between distillers grains and navel orange residue by studying the quality and microbial diversity of the mixed silage.[Method]The crude protein content of distillers grains used in the experiment was 15.05％,and the soluble carbohydrate content of navel orange residue was 24.29％in this study.Seven groups were set up according to a mixed silage proportions(10∶0,9∶1,7∶3,5∶5,3∶7,1∶9,0∶10)of distillers grains and navel orange residue,with 6 repetitions in each group,3 kg for each repetition.The nutritional composition,fermentation quality,and microbial diversity of the silage were measured after 60 days of silage.[Result]The fermentation quality of the mixed silage of distillers grains and navel orange residue showed that the pH of each group was lower than 4.2.Among them,the pH of the 7∶3 and 5∶5 groups were the lowest and significantly lower than those of the 10∶0 and 0∶10 groups(P＜0.05),and the lactic acid content of the 7∶3 and 5∶5 groups were the highest and also significantly higher than these two groups(P＜0.05).The propionic acid content of the 9∶1 and 7∶3 groups were significantly lower than that of the 0∶10 group(P＜0.05),and the butyric acid of which were not detected.Starting from the ratio of mixed silage reaching 7∶3,the ammonia nitrogen content of silage in each group were significantly lower than that of the 10∶0 group(P＜0.05).The measurement results of microbial abundance showed that the total content of five Lactobacillus,including Lactiplantibacillus,Companilactobacillus,Lactobacillus,Limosilactobacillus,Levilactobacillus,in the 7∶3 and 5∶5 groups were significantly higher than that in the 10∶0 group(P＜0.05).The Bacillus abundance of the 7∶3 group was significantly higher than that of the 0∶10 group(P＜0.05).The Pseudomonas abundance of the 9∶1,7∶3,5∶5 and 3∶7 groups were significantly lower than that of the 0∶10 group(P＜0.05).[Conclusion]While the mixed silage proportion for silage of distillers grains and navel orange residue was 7∶3,the fermentation quality and microbial diversity of the silage were better,which was the most appropriate proportion for mixed silage.
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