Effects of Dandelion and Forsythia Extracts on Production Performance,Serum Antioxidant and Immune Indexes of Dairy Cows With Subclinical Mastitis
[Objective]The purpose of this study was to explore the effects of dandelion and Forsythia extracts on production performance,serum antioxidant and immune indexes of dairy cows with subclinical mastitis,in order to provide theoretical basis for the development and application of green feed additives based on plant extract.[Method]18 multiparous Holstein cows with similar body condition,lactation period,milk production,and the mixed milk sample with somatic cell count(SCC)in milk＞5×105/mL were selected.According to the principle of similar SCC,milk samples were divided into control group(CON group),dandelion group(DAN group)and Forsythia group(FOR group).The cows in CON group were fed a total mixed diet(TMR),the cows in DAN group were fed TMR+dandelion extract,and the cows in FOR group were fed TMR+Forsythia extract.None of the cows received any drug treatment within 1 week before the experiment and the experiment lasted for 45 days.Milk samples were collected every 7 days to determine milk composition and SCC.After the experiment,milk samples were collected to determine the activities of bovine myeloperoxidase(MPO),lactate dehydrogenase(LDH)and N-acetyl-β-D-glucosaminase(NAG).Blood samples were collected and serum antioxidant and immune indexs were measured.[Result]Compared with the CON group,① Dietary supplementation of dandelion and Forsythia extract had no significant effect on milk production and milk components of dairy cows with subclinical mastitis(P＞0.05).② The LDH activity in milk of DAN and FOR groups was significantly decreased(P＜0.05).NAG activity in DAN group was significantly decreased(P＜0.05).③ The catalase(CAT)activity in the serum of dairy cows with subclinical mastitis in DAN group was significantly increased(P＜0.05),and the total antioxidant capacity(T-AOC)in FOR group was significantly increased(P＜0.05).Both herbal extracts could significantly reduce the serum malondialdehyde(MDA)content of dairy cows with subclinical mastitis(P＜0.05).④ The contents of IgA,IgG and IgM in serum of dairy cows with subclinical mastitis in DAN and FOR groups were significantly increased(P＜0.05),and the IL-1β content in serum was significantly reduced(P＜0.05).[Conclusion]Dietary supplementation of dandelion and Forsythia extracts could regulate the inflammatory response,enhance the immune and antioxidant capacity of dairy cows with subclinical mastitis,and thus achieve the prevention and control effect of subclinical mastitis in dairy cows.
万方数据
维普
