[Objective]The aim of this study was to investigate the polymorphism of glycerol-3-phosphate acyltransferase 3(GPAT3)gene and its association with growth traits in Jiangquan Black pigs,so as to provide theoretical and technical support for genetic improvement and optimization of the breeding program in Jiangquan Black pigs.[Method]The ear tissues of 292 Jiangquan Black pigs were collected and subjected to DNA extraction.The GPAT3 gene fragment was amplified using PCR,followed by detection of single nucleotide polymorphism(SNP)and genotyping through Sanger sequencing and MassARRAY® nucleic acid mass spectrometry.The genetic effects,linkage disequilibrium and haplotype analysis of GPAT3 gene SNP were analyzed using R 3.6.2,HaploView and PHASE softwares.The association between GPAT3 gene SNP and its LD block with growth traits in Jiangquan Black pigs was assessed using SAS 9.2 software.[Result]A total of 10 SNPs were found in GPAT3 gene and its 5'-regulatory region,of which 4 were low polymorphisms and 6 were moderate polymorphisms.The genotype frequency distribution of 7 SNPs was in Hardy-Weinberg equilibrium.Association analysis found that 9 SNPs were significantly associated with growth traits in Jiangquan Black pigs(P＜0.05),where g.134957929 G＞A,g.134957930 G＞T,g.134958013 G＞A and g.134976983 T＞C had a strong linkage,and the construction of AGGT/AGGT diplotype had significant advantages in chest circumference and body height(P＜0.05),GTGC/GTGC diplotype and GTGC haplotype had significant advantages in body length(P＜0.05).g.135002556 C＞A,g.135002627 C＞T and g.135002680 C＞T had a strong linkage,and the construction of ATC/ATC diplotype had significant advantages in chest and abdominal circumference(P＜0.05),CCC/ATC diplotype had significant advantage in backfat thickness(P＜0.05),and ATT haplotype had significant advantage in abdominal circumference(P＜0.05).[Conclusion]There were abundant polymorphic loci of GPAT3 gene in Jiangquan Black pigs,9 SNPs and 2 haplotype blocks had significant correlation with the growth traits of chest circumference,abdominal circumference and body height,which could be used as molecular genetic markers for growth traits breeding in Jiangquan Black pigs.
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