Transcriptomic Analysis of Host's Bursa of Fabricius After in-ovo Vaccination with Newcastle Disease Vaccine
[Objective]The aim of this experiment was to analyze the transcriptional differences of immune key genes and signaling pathway molecules in host's bursa of Fabricius after in-ovo immunization with Newcastle disease virus TS09-C strain in chicken embryos using transcriptomics,and explore the immune related molecular regulatory responses in the host's bursa of Fabricius after immunization.[Method]Sixty 18 embryo SPF chicken embryos were randomly divided into immunization group and control group,with 30 embryos per group.The immunization group inoculated NDV TS09-C strain into chicken embryos through the amniotic cavity,the dose was 103,0 EID50/piece and the injection volume was 0.1 mL,while the control group was inoculated with equal volume PBS using the same method.The viral load of lung,thymus,spleen and bursa of Fabricius tissue was detected at different time points after immunization.The bursa of Fabricius tissue with the highest viral load was selected for transcriptome sequencing and analysis.Immune related differentially expressed genes were screened and the main functions and pathways they involved were analyzed.[Result]The virus could be detected in bursa of Fabricius tissue 1 day after immunization,and the viral load reached the highest level 3 days after immunization.Transcriptome analysis was performed on the bursa of Fabricius 3 days after immunization.Compared with control group,there were 453 up-regulated genes and 98 down-regulated genes in immunization group,including 36 up-regulated genes and 10 down-regulated genes related to immunity.Immune related differentially expressed genes were mainly enriched in the immune related pathways of autophagy and cytokine-cytokine receptor interactions.Differentially expressed gene protein-protein interaction analysis showed that BCL2 enriched in the autophagy pathway interacted with other immune related genes,and was involved in functional annotation such as B cell activation,differentiation,and immune system development.[Conclusion]The results of this study revealed the potential target genes and pathways regulating the activation and differentiation of B cells in bursa of Fabricius of chicken embryo immunized by NDV TS09-C strain,and provided new information for further study of the molecular mechanism of B lymphocyte regulation after in-ovo vaccination by NDV TS09-C strain.
Newcastle disease virus(NDV)TS09-C strainin-ovo vaccinationbursa of Fabriciustranscriptome analysis
万方数据
维普
