Isolation,Identification and Drug Resistance Analysis of Clostridium perfringens in Calves in a Large-scale Angus Beef Cattle Farm in Kashgar,Xinjiang
[Objective]Clostridium perfringens(CP)is a Gram-positive anaerobic bacterium that can cause sudden calf death,hemorrhagic necrotizing enteritis,enterotoxemia,etc.,which has a huge impact on calf health.The experiment was aimed to understand the prevalence and drug resistance of Clostridium perfringens in Angus calves in a large-scale beef cattle farm in Kashgar prefecture,Xinjiang,so as to provide a scientific basis for the treatment of diseases caused by Clostridium perfringens in this region.[Method]Twenty tissue samples and six enclosure manure samples were collected for isolation and identification of bacteria by separation and culture,morphological observation,staining microscopy,biochemical test,etc.,the drug susceptibility test was carried out by K-B paper method,and the virulence type and drug resistance genes were detected by PCR.[Result]The isolates appeared turbid in FTG liquid medium and produced a large amount of gas,round colonies with neat black edges grew in TSC medium,and colonies with typical double hemolytic rings grew on sheep blood plates.Microscopic examination of Gram staining showed that the bacteria were coarse and short,in single or double arrangement,and the morphology and microscopic examination results were consistent with the characteristics of Clostridium perfringens.A total of 10 strains of Clostridium perfringens were isolated in 26 samples,with a detection rate of 38.46％.After toxin genotyping,9 strains of Clostridium perfringens type A were identified with a detection rate of 90.0％,and 1 strain of Clostridium perfringens type D was identified with a detection rate of 10.0％.Susceptibility test results showed that the isolates had the highest resistance rate of 80.0％to kanamycin,50.0％to gentamicin and lincomycin,and were sensitive to meropenem,cefepime,florfenicol and doxycycline.The results of drug resistance genes showed that the detection rate of blaTEM gene was the highest,which was 100.0％,and the detection rates of blaSHV,qnrA and aac(6')-Ⅰ b-cr resistance genes were 20.0％(2/10),10.0％(1/10)and 20.0％(2/10),respectively,and the two resistance genes of blaCTX-M and qnrS were not detected.[Conclusion]Clostridium perfringens infection in Angus calves in Kashgar prefecture,Xinjiang was mainly type A,with serious drug resistance and multiple drug resistance.The results of this study could provide a scientific basis for epidemiological research and prevention and control of Clostridium perfringens in the later stage.
Angus calvesClostridium perfringensisolation and identificationdrug resistance
国家科技期刊平台
NSTL
万方数据
维普
