[目的]试验旨在研究抗鸭甲肝病毒基因3型(Duck hepatitis A virus genotype 3，DHAV-3)的北京鸭专门化品系(即抗性品系Z7-R)与DHAV-3易感品系(Z7-S)的脂质代谢轮廓，并筛选品系间差异脂质标志物。[方法]选取2日龄Z7系DHAV-3抗性北京鸭和易感北京鸭各6只，采集血液与肝脏组织，进行血液生化指标测定与基于液相色谱-质谱联用技术(LC-MS)的非靶向肝脏脂质组检测。采用t检验、偏最小二乘分析(PLS-DA)和差异倍数(FC)综合筛选品系间显著差异脂质。[结果]Z7-R系北京鸭血浆总胆固醇、低密度脂蛋白和磷脂含量均显著高于Z7-S系(P＜0。05)。脂质组分析共鉴定到1 532个脂质代谢物，涵盖了脂肪酰(FA)、甘油酯(GL)、甘油磷脂(GP)、鞘脂(SP)、固醇脂(ST)5个大类。共筛选到84个显著差异脂质，其中甘油酯类主要为甘油三酯(TG)，且Z7-R系含量均高于Z7-S系(P＜0。05);甘油磷脂类主要为溶血磷脂酰胆碱(LPC)、溶血磷脂酰乙醇胺(LPE)和游离脂肪酸(FFAs)，且Z7-R系含量均低于Z7-S系(P＜0。05)。共筛选到10个显著差异的脂质显著富集到鞘脂代谢、甘油磷脂代谢、甘油酯代谢、亚油酸代谢和α-亚麻酸代谢通路中。[结论]脂质组学技术可用于区分北京鸭Z7-R系与Z7-S系，筛选到的10个显著差异脂质物质可作为潜在的DHAV-3抗性与易感性状相关生物标志物。
Identification and Analysis of Lipid Profile in DHAV-3 Resistant and Susceptible Lines of Pekin Duck
[Objective]The purpose of this experiment was to study the lipid metabolism profiles of the resistant Pekin duck line(Z7-R)and susceptible line(Z7-S)of Pekin ducks to Duck hepatitis A virus genotype 3(DHAV-3),and investigate lipid markers with significant differences between this two lines.[Method]6 2-day-old Z7-R and 6 2-day-old Z7-S Pekin ducks were selected and their blood and liver tissues were collected for blood biochemical index determination and non-targeted liver lipidomics detection based on liquid chromatography-mass spectrometry(LC-MS).t-test,partial least squares-discriminant analysis(PLS-DA)and fold change(FC)were used to comprehensively screen the significantly different lipids between the two lines.[Result]The contents of total cholesterol,low density lipoprotein and phospholipid in the plasma of Z7-R Pekin duck were significantly higher than those in Z7-S(P＜0.05).A total of 1 532 lipid metabolites were identified by lipidome analysis,covering five major categories:Fatty acyl(FA),glycerolipids(GL),glycerophospholipids(GP),sphingolipids(SP),and sterol lipids(ST).A total of 84 significantly different lipids were identified.Triglyceride(TG)was the main component in glycerolipids,and the content of TG in Z7-R Pekin duck was significantly higher than that in Z7-S(P＜0.05).Lysophosphatidylcholine(LPC),lysophosphatidylethanolamine(LPE)and free fatty acid(FFAs)were the significantly different glycerophospholipids,and the contents of these lipids in Z7-R Pekin duck were significantly lower than those in Z7-S(P＜0.05).A total of 10 significantly different lipids were screened and they were significantly enriched in sphingolipid metabolism,glycerophospholipid metabolism,glyceride metabolism,linoleic acid metabolism and α-linolenic acid metabolism.[Conclusion]Lipidomics technology could be used to differentiate Z7-R lines from Z7-S lines of Beijing ducks.The 10 significantly different lipids could be used as potential biomarkers related to DHAV-3 resistant and susceptible traits.
万方数据
维普
