Effects of Feeding Risperidone During Late Pregnancy on Mammary Gland Development of Maternal Mice and Growth of Offspring Mice
[Objective]In this study,mice were used as experimental objects to explore whether risperidone could promote mammary gland development and offspring growth in mammals.[Method]Twenty Kunming female mice were divided into two groups with 10 mice in each group.Mice in experimental group were fed with risperidone containing 0.25 mg/L in the later stage of pregnancy for 4 days,and mice in control group were fed with drinking water without risperidone.Mammary gland tissue and blood were collected from 5 mice in each group on the 3rd and 18th day of lactation.PRL levels in serum were detected by ELISA,breast tissues were analyzed by HE staining and transcriptome sequencing techniques,the differences of breast weight of maternal mice,and average weight and survival rate of offspring mice between two groups were calculated by weighing and counting methods,and GO,KEGG and GSEA enrichment analysis were performed on the sequencing data.[Result]On the 3rd day of lactation,PRL levels in serum of risperidone-treated maternal mice were extremely significantly higher than that of control group(P＜0.01).The mammary gland richness on the 3rd and 18th day of lactation risperidone-treated maternal mice was higher than that of control group.On the 18th day of lactation,breast weight of risperidone-treated maternal mice,average weight and survival rate of offspring mice were significantly higher than that of control group(P＜0.05).GO enrichment analysis showed that the differentially expressed genes(DEGs)on the 3rd day of lactation were mainly enriched in biological processes such as fatty acid metabolism and adipocyte differentiation,and the DEGs on the 18th day of lactation were mainly enriched in biological processes such as negative regulation of fatty acid metabolism and tissue remodeling.KEGG pathway enrichment analysis showed that the DEGs on the 3rd day of lactation were mainly enriched in the PPAR signaling pathway,cAMP signaling pathway,fat digestion and absorption and other pathways,while the DEGs on the 18th day of lactation were mainly enriched in the PPAR signaling pathway,regulation of adipocyte lipolysis and other pathways.GSEA showed that the estrogen and PRL signaling pathways were enriched in the risperidone-treated maternal mice in both periods.[Conclusion]Feeding risperidone to female mice in late pregnancy could promote the development of maternal mammary glands and growth of offspring mice.This study revealed the promoting effect of risperidone on mammary gland development in mice,which laid the foundation for the establishment of a new method of adding risperidone to the drinking water of female animals in the later stage of pregnancy to improve female milk production and offspring growth.It was of great significance to promote the reproduction and breeding efficiency of mammals.
万方数据
维普
