[Objective]The aim of this study was to investigate the effect of the polymorphism of keratin 10(KRT10)gene on reproductive traits in Kele pigs,in order to improve the fertility of Kele pigs.[Method]There were 255 Kele pigs used in this experiment.The single nucleotide polymorphism(SNP)of KRT10 gene was identified using a combination of PCR product sequencing,DANMAN sequence alignment software and manual verification.The population genetic characteristics of SNP were analyzed by SHEsis online software,and the bioinformatics analysis of SNP was conducted by RNAfold online tool.The general linear model of SPSS 22.0 software was used to analyze the correlation between SNP of KRT10 gene and reproductive traits in Kele pigs.[Result]Three SNPs were identified in KRT10 gene of Kele pigs:g.21643703 C＞T and g.21643714 G＞A in intron 4,and g.21643741 G＞A in exon 5.g.21643741 G＞A mutation caused a codon mutation from AAG to AAA,encoding amino acids of lysine(K),which was a synonymous mutation and caused a change of the secondary structure in mRNA.Three genotypes were detected at all three mutation sites.g.21643703 C＞T and g.21643741 G＞A belonged to moderate polymorphism sites(0.25＜PIC＜0.5),and g.21643714 G＞A belonged to low polymorphism site(PIC＜O.25).g.21643703 C＞T and g.21643741 G＞A were in Hardy-Weinberg equilibrium(P＞0.05),g.21643714 G＞A was significantly deviated from Hardy-Weinberg equilibrium(P＜0.05).There were no strong linkage imbalance among three SNPs.Three SNPs produced a total of 4 haplotypes and 10 diploids.The results of correlation analysis showed that the effect of g.21643703 C＞T on newborn litter weight,weaned piglets and weaned litter weight in Kele pigs reached a significant level(P＜0.05),the effect of g.21643714 G＞A on total litter size and weaned litter weight in Kele pigs reached a significant level(P＜0.05),and the effect of g.21643741 G＞A on total litter size,live litter size,weaned piglets and weaned litter weight in Kele pigs reached a significant level(P＜0.05).The diploid H3H3 had the most significant effects on total litter size,live litter size,weaned piglets and weaned litter weight in Kele pigs.[Conclusion]Three SNPs of KRT10 gene had significant effects on reproductive traits in Kele pigs,and H3H3 was advantageous diplotype,which might be used as a genetic marker for reproductive trait selection in Kele pigs.
关键词
柯乐猪/KRT10基因/单核苷酸多态性(SNP)/繁殖性状
Key words
Kele pigs/KRT10 gene/single nucleotide polymorphism(SNP)/reproductive traits
维普
万方数据