Genetic Diversity and Phylogenetic Analysis of Goat Population in Karakoram-Pamir Region
[Objective]This experiment was conducted to further clarify the genetic diversity and phylogeny of goat population in Karakoram-Pamir region,and to provide theoretical basis for the conservation,excavation and application of biological resources.[Method]The mitochondrial(mtDNA)D-loop region and cytochrome b(Cytb)gene sequences were amplified by PCR and sequenced of goats in Karakoram-Pamir region.Mega 7.0.26 software was used to analyze the base composition and draw the phylogenetic tree by combining the D-loop region and Cytb gene of goats downloaded from GenBank,and DNASP 5.10 software was used to analyze haplotype distribution and neutral test,and haplotype media-joining network was constructed by Network 10.2 software.[Result]The AT content in mtDNA D-loop region and Cytb gene sequences of goats in Karakoram-Pamir region was 60.1％and 58.7％,respectively,and the AT content was higher than the GC content,showing a preference for base composition.There were 163 SNPs in D-loop region and 84 SNPs in Cytb gene,and 40 and 30 haplotypes were defined,respectively,with haplotype diversity(Hd)of 0.985 and 0.883 and nucleotide diversity(Pi)of 0.01570 and 0.00425,respectively.The Tajima's D value and Fu's Fs value of mtDNA D-loop region and Cytb gene were all less than 0 by the neutrality test,and significantly deviate from neutrality.Phylogenetic tree analysis showed that goats in Karakoram-Pamir region were distributed in 4 lineages:A,B,C and G.[Conclusion]Goats in Karakoram-Pamir region had experienced at least three population expansion events and had rich genetic diversity.There was gene infiltration from goats in Western Asia,while Capra ibexs in the same region had no genetic contribution.
万方数据
维普
