Detection of Drug Resistance of Escherichia coli from Ducks in Some Areas of Tongliao and Whole Genome Sequencing Analysis of Multi-drug Resistant Strain YA-1
Detection of Drug Resistance of Escherichia coli from Ducks in Some Areas of Tongliao and Whole Genome Sequencing Analysis of Multi-drug Resistant Strain YA-1
[Objective]As one of the most common bacterial pathogens in poultry,the increasing resistance of Escherichia coli(E.coli)has attracted wide attention.The purpose of this study was to understand the distribution of drug resistance and drug resistance genes of E.coli in some duck farms in Tongliao area,so as to provide reference for the study of drug resistance and drug resistance genes of E.coli from ducks in this area.[Method]The pathogenic bacteria were isolated and purified from 15 intestinal samples of dead ducks in each county of Tongliao city by 16S rRNA sequencing,biochemical test and mouse pathogenicity test.The isolated strains were subjected to drug sensitivity test and PCR detection of drug resistance genes,and multi-drug resistant strains were selected for whole genome sequencing.[Result]All 10 strains of E.coli had different degrees of drug resistance.The resistance rates to streptomycin,ciprofloxacin,ofloxacin and enrofloxacin were all above 80％,and the detection rates of drug resistance genes aphA1,strB and qnrS were 100％.The whole genome sequencing of the multi-drug resistant strain YA-1 confirmed that the chromosome size of the strain was 4 844 827 bp,carrying 3 complete plasmids,which were 144 776 bp(pTLYA-1),113 584 bp(pTLYA-2)and 77 544 bp(pTLYA-3),respectively.The isolated strains carried 70 drug resistance genes such as arr-3,aadA16 and sul1.Among them,pTLYA-1 carried 8 mobile drug resistance genes such as arr-3,dfrA27 and aadA16,pTLYA-3 carried 5 mobile drug resistance genes such as floR,tetA and sul2,and no drug resistance genes were found in pTLYA-2.[Conclusion]E.coli isolated from some duck farms in Tongliao area had multiple drug resistance,and the resistance level was high.The resistance genes of aphA1,strB and qnrS were prevalent.
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