[目的]研究中药组方对犊牛源大肠杆菌致小鼠腹泻的治疗效果，为中药治疗大肠杆菌致犊牛腹泻提供参考依据。[方法]将162只小鼠随机分为9组，空白组、模型组、中药治疗组(2个组方各3个浓度(1。5、1。0、0。5 g/mL))和西药治疗组，每组3个重复，每个重复6只。除空白组外，其余各组小鼠腹腔注射0。5 mL 1×108 CFU/mL的大肠杆菌悬液，小鼠出现腹泻后，中药组小鼠分别灌喂0。2 mL不同浓度中药组方提取液(白头翁汤加减和乌梅散加减)，西药组小鼠灌喂0。2 mL恩诺沙星，模型组小鼠灌喂0。2 mL蒸馏水，持续7 d。每天记录小鼠体重、采食量，治疗第3天时统计腹泻指数;治疗第7天剖检小鼠，检测小肠病理变化，利用ELISA法检测回肠黏膜分泌型免疫球蛋白(sIgA)、多聚免疫球蛋白受体(pIgR)含量，利用实时荧光定量PCR检测十二指肠Claudin-1、Occludin和ZO-1基因mRNA相对表达量。[结果]与模型组相比，经2个中药组方治疗后腹泻小鼠体重、采食量均可显著增加(P＜0。05)，腹泻指数显著降低(P＜0。05)，改善了小肠病理组织学病变，促进了肠绒毛损伤的修复。与模型组相比，2个组方均可显著提高小鼠肠黏膜sIgA、pIgR含量(P＜0。05)及十二指肠Claudin-1、Occludin和ZO-1基因mRNA相对表达量(P＜0。05)。1。0 g/mL乌梅散加减在2个中药组方中治疗效果最佳，与西药组相比，在小鼠肠组织形态、肠黏膜免疫球蛋白含量及紧密连接蛋白mRNA的相对表达量上不存在显著差异。[结论]乌梅散加减和白头翁汤加减均可降低小鼠腹泻指数，改善肠道病理损伤，其中乌梅散加减的治疗效果更显著，本研究可为中药治疗大肠杆菌致犊牛腹泻提供参考。
Study on the Therapeutic Effect of Traditional Chinese Medicine Formula on Diarrhoea Caused by Escherichia coli in Mice
[Objective]The aim of this experiment was to study the therapeutic effect of traditional Chinese medicine formulas on calf-derived Escherichia coli-caused diarrhoea in mice,and to provide a reference basis for the treatment of Escherichia coli-caused diarrhoea in calves with traditional Chinese medicine.[Method]162 mice were randomly divided into 9 groups,blank group,model group,traditional Chinese medicine treatment groups 2 formulas(with 3 concentrations(1.5,1.0 and 0.5 g/mL)for each)and Western medicine treatment group,with 3 replicates in each group and 6 mice in each replicate.Except for blank group,the mice in each group were injected intraperitoneally with 0.5 mL Escherichia coli suspension with a concentration of 1×108 CFU/mL,and after diarrhoea appeared in mice,the test mice in traditional Chinese medicine group were gave 0.2 mL extracts of traditional Chinese medicine formulas(Bai Tou Weng Tang plus or minus and Wu Mei San plus or minus),respectively,and the test mice in Western medicine group were gave 0.2 mL enrofloxacin.In model group,mice were fed 0.2 mL distilled water.The test lasted for 7 d.The body weight and food intake were recorded daily,and the diarrhoea index was counted on the 3rd day of treatment,mice were dissected on the 7th day of treatment,and pathological changes in the small intestine were detected,and the content of ileal mucosal immunoglobulins,sIgA and pIgR were detected using ELISA,and duodenal Claudin-1,Occludin and ZO-1 genes mRNA relative expression was detected using Real-time quantitative PCR.[Result]Compared with model group,treatments with two herbal formulas could significantly increase the body weight and food intake of diarrhoeic mice,significantly reduce the diarrhoea index(P＜0.05),improve the pathological and histological lesions of the small intestine,and promote the repair of intestinal villi damage.Compared with model group,both formulas could significantly enhance the contents of mouse intestinal mucosal immunoglobulin sIgA and pIgR(P＜0.05),and significantly enhance the relative mRNA expression of mouse duodenal Claudin-1,Occludin and ZO-1 genes mRNA relative expression(P＜0.05).1.0 g/mL Wu Mei San plus or minus had the best therapeutic effect among the two traditional Chinese medicine formulas,and had no significant difference in intestinal histomorphology,intestinal mucosal immunoglobulin content and relative mRNA expression of tight junction proteins compared with Western medicine group.[Conclusion]The addition and subtraction of Wu Mei San plus or minus and Bai Tou Weng Tang plus or minus could reduce the diarrhoea index and improve the pathological damage of the intestinal tract in mice,among which the therapeutic effect of the addition and subtraction of Wu Mei San plus or minus was more significant,and this study could provide reference for the treatment of diarrhoea of calves caused by Escherichia coli by traditional Chinese medicine.
Escherichia colimousetraditional Chinese medicinetreatmentcalf diarrhoea
万方数据
维普
