Exploring Candidate Genes Related to Kidney-adjacent Lipid Deposition in Yak Calves Based on Transcriptome Sequencing
[Objective]This study was aimed to compare the changes in transcriptome expression profiles of kidney-adjacent adipose tissue in yak calves at different ages,and explore the signaling pathways and key genes that regulated kidney-adjacent lipid deposition in yak calves.[Method]Three healthy male yak calves at 1,7 and 30 day-old were bled to death,and the kidney-adjacent adipose tissue were collected for transcriptome sequencing using Illumina HiSeq platform,after which the transcriptome data were subjected to quality control,comparison,differentially expressed gene(DEGs)screening,and the DEGs were performed to GO function and KEGG pathway enrichment analysis.In addition,seven genes were randomly selected for Real-time quantitative PCR verification.[Result]Transcriptome results showed that a total of 1 627 significantly DEGs were identified in the comparison group of 1-and 7-day-old,of which 939 were up-regulated and 688 were down-regulated.Also,a total of 3 979 significantly DEGs were identified in the comparison group of 7-and 30-day-old,of which 1 925 were up-regulated and 2 054 were down-regulated.GO function and KEGG pathway enrichment analysis showed that the DEGs were enriched in acyl-CoA dehydrogenase activity to participate in fatty acid biosynthesis,insulin resistance,PPAR signaling pathway,sphingolipid signaling pathway,etc.in the comparison group of 1-and 7-day-old.In addition,the DEGs were enriched in several biological processes,including fatty acid metabolism,lipid metabolism,phosphohydrolase activity and lipase activity to participate in butyrate metabolism,fatty acid metabolism,glycerophospholipid metabolism,phospholipase D signaling pathway,AMPK signaling pathway,etc.in the comparison group of 7-and 30-day-old.The enriched pathways showed that SCD,ADIPOQ,PLIN1,ACADM and LPL were identified as candidate genes associated with kidney-adjacent lipid deposition in yak calves.Real-time quantitative PCR results showed that the expression of seven genes were consistent with RNA-Seq results.[Conclusion]Five genes related to adipocyte differentiation and fat deposition were screened in this study,including SCD,ADIPOQ,PLIN1,ACADM and LPL.The results provided a theoretical reference for further research on kidney-adjacent lipid deposition in yak calves at different growth stages.
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