Effect of Cepharanthine on Anti-apoptotic Ability of Bovine Oocytes and Early Embryos in vitro
[Objective]This study was aimed to explore the effect of cepharanthine(Ceph)on the antiapoptotic capacity of bovine oocytes and early embryos,so as to provide reference for improving the efficiency of bovine embryo production in vitro and new data for the pharmacological action of Ceph in anticancer process.[Method]Bovine oocytes were cultured in in vitro mature medium containing different concentrations(0,10,100,500 and 1 000 μmol/L)of Ceph to observe the oocyte maturation rate and early embryo development after fertilization,the optimal concentration of Ceph was determined.The cathepsin B(CB)activity,the cell autophagy-related protein(LC3)level,the mRNA expression of apoptosis-related genes Survivin and Caspase-3,and the apoptosis rate of blastocysts in bovine oocyte were detected.[Result]The maturation rate of bovine oocytes and the division rate of early embryo treated with 500 μmol/L Ceph were significantly higher than that of other groups(P＜0.05).The addition of Ceph significantly reduced the CB activity and LC3 level in bovine oocytes(P＜0.05),significantly increased the mRNA expression of Survivin gene(P＜0.05),and significantly reduced the mRNA expression of Caspase-3 gene and the apoptosis rate of blastocysts(P＜0.05).[Conclusion]The addition of Ceph during in vitro embryo production could regulate the expression of apoptosis-related genes Survivin and Caspase-3 to affect the levels of CB and LC3,and enhance the anti-apoptotic capacity of bovine oocytes and in vitro embryo production efficiency.
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