Effect of Different Factors on the Infection of Gastrointestinal Nematodes in Teha Crossbred Sheep
[Objective]This experiment was aimed to study the effects of season,gender,group and flock on the infection intensity of gastrointestinal nematodes(GIN),weight,hematocrit and specific antibody immunoglobulin G(IgG)content in Texel and Kazakh(hereinafter referred to as Teha)crossbred sheep,in order to provide a reference basis for research on the prevention and treatment of GIN in sheep and anti-GIN breeding.[Method]Feces were collected rectally,blood was collected from the jugular vein,and weights were weighed in Teha crossbred sheep.The eggs of GIN were qualitatively detected by saturated brine flotation method.The modified McMaster method was used for egg counting of GIN,Infection rate and infection intensity were calculated.Hematocrit was measured using a veterinary auto hematology analyzer.Nematode L3 larval somatic cell antigens were prepared,and specific antibody IgG content in the serum of the sheep was determined using indirect ELISA method.[Result]The infection rate of GIN in Teha crossbred sheep was high at 94.44％,which was moderate.And total of nine GIN were identified which were Trichostrongylus spp.,Haemonchus contortus,Ostertagia spp.,Bumastomum trigonocephalum,Chabertia spp.,Marshallagia spp.,Nematodirus spp.,Oesophagostomum spp.and Trichuris globuulosa.Season had a significant effect on the infection intensity of GIN and weight of sheep(P＜0.05),the infection intensity of GIN was significantly higher in spring than that in summer(P＜0.05),and the weight of sheep was significantly higher in summer than that in spring(P＜0.05).Gender had a significantly effect on the infection intensity,weight and hematocrit(P＜0.05),with the infection intensity of GIN significantly higher in rams than in ewes(P＜0.05),the weight was significantly higher in rams than in ewes(P＜0.05),and hematocrit was significantly higher in ewes than in rams(P＜0.05).Group had a significant effect on weight(P＜0.05),the weight of F2XHa sheep was significantly higher than that of F2 and F2XF1 sheep(P＜0.05),the weight of F1×F1 sheep was significantly higher than that of F2XF1 sheep(P＜0.05),and the weight of F2×F2 sheep was significantly lower than that of other groups(P＜0.05).Flock had a significant effect on the infection intensity in sheep(P＜0.05),with the infection intensity of GIN in flock 1 being significantly higher than that in flocks 2 and 3(P＜0.05),and the infection intensity of GIN in flock 3 was significantly higher than that in flock 2(P＜0.05).All factors had no effect on specific antibody IgG content(P＞0.05).[Conclusion]Season,gender,group and flock had influence on the infection of GIN in sheep,and different control measures could be selected for sheep through the effect of factors on the infection and the differences between different levels of each factor,to provide a theoretical basis for anti-GIN breeding strategies.
Teha crossbred sheepgastrointestinal nematodesinfection rateintensity of infection
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