Evaluation of the Protective Effect of Lycopene on Organ Injury Induced by Cisplatin in Rats
[Objective]The aim of this study was to evaluate the protective effect of lycopene(LCP)on organ injury induced by cisplatin(CDDP)in rats.[Method]Thirty male Wistar rats were randomly divided into 5 groups(n=6),which were control group(CON),CDDP and different LCP treatment groups(5 mg/kg,LLCP group;10 mg/kg,MLCP group;50 mg/kg,HLCP group).Rats in CON and CDDP groups were given 1 mL/kg corn oil per day,and rats in LLCP,MLCP and HLCP groups were given the corresponding concentration of LCP per day,and consecutive for 28 days.Except CON group,the other groups were given a single intraperitoneal injection of CDDP(7 mg/kg)on day 26.After the experiment,samples of stomach,small intestine(duodenum,jejunum and ileum),liver,kidney and blood were collected.The protective effects of different concentrations of LCP on CDDP-induced acute injury in rats were evaluated by physiological and biochemical indexes detection,HE staining,ELISA and Western blotting.[Result]Compared with CON group,body weight,feed intake and water intake of rats in CDDP group were extremely significantly or significantly decreased(P＜0.01 or P＜0.05).Serum levels of aspartate aminotransferase(AST),urea nitrogen(BUN),creatinine(CRE)and malondialdehyde(MDA),interleukin-8(IL-8),IL-6 and tumor necrosis factor-α(TNF-α)were significantly or extremely significantly increased(P＜0.05 or P＜0.01).The activities of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)were extremely significantly decreased(P＜0.01),and the histopathological injuries of stomach,small intestine,liver and kidney were serious.Compared with CDDP group,the body weight,feed intake and water intake of rats in MLCP group were significantly increased(P＜0.05),the levels of 6 biochemical indexes such as AST and ALT and the contents of MDA,IL-8,TNF-α and IL-6 in serum were significantly decreased(P＜0.05),and the organ histomathological damage was alleviated.Western blotting results showed that compared with CON group,the expression of TNF-α and IL-6 proteins in different tissues of rats in CDDP group were extremely significantly increased(P＜0.01),while the expression of IL-10 protein was extremely significantly decreased(P＜0.01).Compared with CDDP group,the expression of TNF-α and IL-6 proteins in all tissues of MLCP group were extremely significantly or significantly decreased(P＜0.01 or P＜0.05),the expression of IL-10 protein was extremely significantly or significantly increased(P＜0.01 or P＜0.05).[Conclusion]LCP could improve the stomach,small intestine,liver and kidney injury in CDDP-induced rats by inhibiting oxidative stress and inflammation.Under this experiment conditions,10 mg/kg LCP had the best effect.
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