Response of Fattening Hu Sheep to Cecropin Antimicrobial Peptides
[Objective]The aim of this study was to investigate the effects of adding cecropin antimicrobial peptide to diet on the growth performance,serum immune indicators,antioxidant function,and rumen microbiota structure of Hu sheep.[Method]36 5-month-old male Hu sheep with similar body weight(33.47 kg±1.24 kg)were randomly divided into 2 groups with 3 replicates per group and 6 sheep per replicate.The sheep in control group(CON)was fed with basic feed,and in antimicrobial peptide group(AMP)was supplemented with 0.5 g/kg of cecropin antimicrobial peptide in basal diet.After a 30 day feeding experiment,serum and rumen fluid samples were collected,and the growth performance,serum immunoglobulin,cytokine content,antioxidant function,and rumen fluid microbial community structure of sheep were determined.[Result]The final weight and average daily gain(ADG)of Hu sheep in AMP group were significantly higher than those in CON group(P＜0.05),while feed-to-gain ratio(F/G)was significantly lower than that in CON group(P＜0.05).The levels of immunoglobulin G(IgG),IgM,superoxide dismutase(SOD),and total antioxidant capacity(T-AOC)in serum of Hu sheep in AMP group were significantly higher than those in CON group(P＜0.05).Cytokines interleukin-6(IL-6)and tumor necrosis factor-alpha(TNF-α)of Hu sheep in AMP group were significantly lower than those in CON group(P＜0.05).The addition of cecropin antimicrobial peptides to the diet did not significantly affect the rumen microbiota richness indices Sobs index,Chao1 index,and Ace index,as well as diversity indices Shannon index and Simpson index in Hu sheep(P＞0.05).Relative abundances of Proteobacteria and Christensenellaceae_R-7_group in the rumen of Hu sheep in AMP group were significantly lower than those in CON group,while relative abundances of Bacteroidales_RF16_group,norank_f__F082,and NK4A214_group were significantly higher than those in CON group(P＜0.05).[Conclusion]Adding 0.5 g/kg of cecropin antibacterial peptide to the diet was beneficial for improving the growth performance of fattening Hu sheep,enhancing immune and antioxidant performance,reducing the secretion level of inflammatory factors,and the abundance of harmful bacteria in the rumen,regulating the structure of rumen microorganisms.
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