Identification and Function Prediction of lncRNA Related to Reproductive Function of Uterine Body in Tibetan and Chuanxiang Black Pigs During Pregnancy
[Objective]This study was aimed to investigate the regulatory mechanism of long non-coding RNA(lncRNA)in reproduction of Tibetan and Chuanxiang Black pigs,and screen the related lncRNA.[Method]The pregnant uterine body samples from Tibetan and Chuangxiang Black pigs with different litter sizes were collected,a specific porcine uterine body mRNA library was established,and sequencing was performed on the Illumina PE150 HiSeq platform.After quality control,alignment and splicing,the differentially expressed lncRNA was screened and its target gene was predicted.GO function and KEGG pathway enrichment analysis were performed on the target genes.In addition,7 differentially expressed lncRNAs were randomly selected for Real-time quantitative PCR verification.[Result]There were 32 co-differentially expressed lncRNAs in uterine body of Tibetan and Chuanxiang Black pigs,and a total of 168 target genes were obtained.GO function analysis showed that the target genes of co-differentially expressed lncRNAs in samples with different reproductive ability were mainly enriched in items such as molecular metabolism,transcriptional activity regulation,cell proliferation regulation,uterine body modification,trace element metabolism,etc.KEGG pathway analysis showed that the target genes of co-differentially expressed lncRNAs were mainly enriched in glycolysis,oocyte division,cell life regulation,Ras signaling pathway,RIG-Ⅰ signaling pathway,FoxO signaling pathway,HIF-1 signaling pathway,regulation of homeostasis process,etc.Real-time quantitative PCR results showed that the expression of 7 differentially expressed lncRNAs were consistent with RNA-Seq results.[Conclusion]This study found 32 lncRNAs in uterine body of Tibetan and Chuanxiang Black pigs that might be involve in the pregnancy process of sows by regulating potential target genes,so as to provide a reference basis for further research on the regulatory mechanism of lncRNA in sow reproduction.These lncRNAs could be used as new reproductive trait biomarkers for the breeding process of pigs.
Tibetan pigsChuanxiang Black pigsuterine bodypregnancylong non-coding RNA(lncRNA)
国家科技期刊平台
NSTL
万方数据
